Institute of Forensic Science, Wenzhou Medical University, Wenzhou 325000, China.
Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Biomed Res Int. 2020 Dec 4;2020:8844866. doi: 10.1155/2020/8844866. eCollection 2020.
In this paper, a UPLC-MS/MS method was developed for the determination of ropivacaine and its metabolite 3-hydroxy ropivacaine in cerebrospinal fluid. The cerebrospinal fluid was processed by ethyl acetate liquid-liquid extraction. The multiple reaction monitoring (MRM) mode was used for quantitative analysis by monitoring the transitions of m/z 275.3 → 126.2 for ropivacaine, m/z 291.0 → 126.0 for 3-hydroxy ropivacaine, and m/z 290.2 → 198.2 for the internal standard. Standard curves for ropivacaine and 3-hydroxy ropivacaine in cerebrospinal fluid were conducted over the concentration range of 0.2-2000 ng/mL, demonstrating excellent linearity, and the lower limit of quantification was 0.2 ng/mL. The intraday precision of ropivacaine and 3-hydroxy ropivacaine was less than 11%, while the interday precision was less than 7%. The accuracy ranged between 87% and 107%, the average extraction efficiency was higher than 79%, and the matrix effect was between 89% and 98%. The developed method was then applied to a case of suspected poisoning of ropivacaine.
本文建立了一种 UPLC-MS/MS 法，用于测定脑脊液中的罗哌卡因及其代谢产物 3-羟罗哌卡因。采用乙酸乙酯液液萃取法处理脑脊液。采用多反应监测（MRM）模式，通过监测 m/z 275.3→126.2（罗哌卡因）、m/z 291.0→126.0（3-羟罗哌卡因）和 m/z 290.2→198.2（内标）的离子对进行定量分析。罗哌卡因和 3-羟罗哌卡因在脑脊液中的标准曲线浓度范围为 0.2-2000ng/mL，表现出良好的线性，定量下限为 0.2ng/mL。罗哌卡因和 3-羟罗哌卡因的日内精密度均小于 11%，日间精密度均小于 7%。准确度在 87%至 107%之间，平均提取效率高于 79%，基质效应在 89%至 98%之间。该方法随后应用于一例疑似罗哌卡因中毒的案例。
