Department of Clinical Biochemistry, Vendsyssel Hospital, Bispensgade 37, DK-9800 Hjørring, Denmark.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):76-82. doi: 10.1016/j.jchromb.2009.11.028.
A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method, using back-flush column-switching was developed for total drug concentrations of ropivacaine in serum and drainage blood in the measuring range 0.1-10microg/mL. Samples were diluted with internal standard ((2)H(7)-ropivacaine) and extraction buffer, centrifuged and injected directly onto a BioTrap 500 MS extraction column. Using a time programmed six-port valve switch, ropivacaine was back-flushed onto a Zorbax SB-Aq analytical column, gradient eluted and finally detected after electro spray ionisation and multiple reaction monitoring (MRM) of the transitions m/z 275-->m/z 126 and m/z 282-->m/z 133 for ropivacaine and (2)H(7)-ropivacaine, respectively. Accuracy (bias-%) was -1.5 to 5.8% and intermediate precision (C.V.) was 1.4-3.1%. The low sample amount required (10microL), high specificity and short run time (6 min) makes it very suitable for determination of ropivacaine. Using the same methodology as described above and 200microL ultrafiltrate, the free drug concentrations of ropivacaine in serum could be precisely determined with a C.V. below 3%. The method was used to investigate the safety of reinfusion of drainage blood after knee and hip arthroplasty when ropivacaine (Naropin) was used for local analgesia. Data for 30 patients are summarised.
一种高效液相色谱-串联质谱（HPLC-MS-MS）法，采用反冲洗柱切换技术，用于测量血清和引流血液中罗哌卡因的总药物浓度，测量范围为 0.1-10μg/mL。样品用内标（（2）H（7）-罗哌卡因）和提取缓冲液稀释，离心后直接注入 BioTrap 500 MS 提取柱。使用时间编程六通阀切换，将罗哌卡因反冲洗到 Zorbax SB-Aq 分析柱上，梯度洗脱，最后通过电喷雾电离和多重反应监测（MRM）进行检测，用于罗哌卡因和（2）H（7）-罗哌卡因的转换 m/z 275->m/z 126 和 m/z 282->m/z 133。准确度（偏差-％）为-1.5 至 5.8％，中间精密度（C.V.）为 1.4-3.1％。所需的低样品量（10μL）、高特异性和短运行时间（6 分钟）使其非常适合罗哌卡因的测定。使用与上述相同的方法和 200μL 超滤物，可以精确测定血清中罗哌卡因的游离药物浓度，C.V.低于 3％。该方法用于研究膝关节和髋关节置换术后引流血再输注的安全性，当时罗哌卡因（Naropin）用于局部镇痛。总结了 30 名患者的数据。
