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.中线粒体基因组的完整序列测定与分析

Complete sequence determination and analysis of mitochondrial genome in .

作者信息

Deng Huaiqing, Wen Huamei, Xiao Ning, Zhou Jiang

机构信息

School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, China.

Guiyang Nursing Vocational College, Guiyang, Guizhou, China.

出版信息

Mitochondrial DNA B Resour. 2016 Sep 5;1(1):590-592. doi: 10.1080/23802359.2016.1202739.

Abstract

revealed that the complete length of its mitochondrial genome was 16,578 bp, composed of A (30.7%), T (25.6%), G (16.2%), C (27.5%) and A + T (56.3%). Its genetic constitution and arrangement were consistent with those of other Osteichthyes, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control area (D-loop). All genes were encoded by the H-strand, except for 1 protein-coding gene (ND6) and 8 tRNA genes (tRNA-Gln, tRNA-Ala, tRNA-Cys, tRNA-Asn, tRNA-Tyr, tRNA-Ser, tRNA-Glu and tRNA-Pro) are encoded by the L-strand. The mitochondrial genes were arranged very closely. There were four gene overlapping regions, with an overall length of 21 bp and a base covering number range of 1-14 bp, and 23 intergenic regions, with an overall length of 147 bp and an intergenic length range of 1-19 bp. There were 10 gene pairs that were neither overlapping nor intergenic.

摘要

结果表明,其线粒体基因组全长16578bp,由A(30.7%)、T(25.6%)、G(16.2%)、C(27.5%)组成,A+T含量为56.3%。其基因组成和排列与其他硬骨鱼一致,包括13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区(D-loop)。除1个蛋白质编码基因(ND6)和8个tRNA基因(tRNA-Gln、tRNA-Ala、tRNA-Cys、tRNA-Asn、tRNA-Tyr、tRNA-Ser、tRNA-Glu和tRNA-Pro)由轻链编码外,所有基因均由重链编码。线粒体基因排列非常紧密。有4个基因重叠区域,总长21bp,碱基覆盖数范围为1-14bp,23个基因间隔区域,总长147bp,基因间隔长度范围为1-19bp。有10对基因既不重叠也不间隔。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23af/7801021/9fc15ec4928d/TMDN_A_1202739_F0001_B.jpg

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