Wang Zhongduo, Long Shuisheng, Liao Jian, Huang Chengqin, Zhang Hairui, Huang Shunkai, Zhang Yanping, Liu Li, Guo Yusong
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Mitochondrial DNA B Resour. 2017 Mar 21;2(1):157-158. doi: 10.1080/23802359.2017.1303340.
In this study, combining the liver transcripts from both sexes by RNA-Seq with DNA sequences by conventional PCRs, we have determined the complete mitogenome of Hainan medaka collected from the mangrove seawater of the Leizhou Peninsula in tropical South China (Accession no.: KY364884). The mitochondrial genome is 16,676 bp, and its content and structure are highly homologous to those of other teleostean fishes, including 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNA and 1 control region. Among the PCGs, ATG is used as the initiation codon, except for GTG in COI gene. There are 7 overlapping genes with overlap lengths ranging from 1 to 10 nucleotides (nt), while ten intergenic regions with a total of 66 nt and a maximum interval of 37 nt between tRNA and tRNA. Moreover, the data from RNA-Seq shows that the significant differences exist in the expression patterns of mitogenomes between male and female.
在本研究中，我们通过RNA测序将两性的肝脏转录本与常规聚合酶链式反应（PCR）得到的DNA序列相结合，确定了从中国热带南部雷州半岛红树林海水中采集的海南青鳉的完整线粒体基因组（登录号：KY364884）。该线粒体基因组为16,676 bp，其含量和结构与其他硬骨鱼类高度同源，包括13个蛋白质编码基因（PCG）、2个核糖体RNA（rRNA）、22个转运RNA（tRNA）和1个控制区。在这些蛋白质编码基因中，除COI基因使用GTG作为起始密码子外，其余均使用ATG作为起始密码子。有7个重叠基因，重叠长度在1至10个核苷酸（nt）之间，同时有10个基因间隔区，总长66 nt，tRNA与tRNA之间的最大间隔为37 nt。此外，RNA测序数据表明，线粒体基因组在雄性和雌性中的表达模式存在显著差异。
