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(硬骨鱼纲:颌针鱼目)的完整线粒体基因组及系统发育分析

Complete mitogenome and phylogenetic analysis of (Teleostei: Beloniformes).

作者信息

Ma Jiangru, Long Shuisheng, Wang Zhongduo

机构信息

Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, China.

出版信息

Mitochondrial DNA B Resour. 2019 Dec 9;5(1):27-28. doi: 10.1080/23802359.2019.1695550.

DOI:10.1080/23802359.2019.1695550
PMID:33366406
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7721023/
Abstract

The complete mitogenome was determined using next-generation sequencing (NGS) method and the resulting data analyzed in this article. The mitochondrial genome was 16,493 base pair (bp) length, and its content and structure were highly homologous to that of other teleostean fishes, including 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNA, and 1 control region. Among the PCGs, ATG was used as the initiation codon, except for GTG in the COI gene. There were 6 overlapping genes with overlap lengths ranging from 1 to 10 nucleotides (nt), while 10 intergenic regions with a total of 59 nt and a maximum interval of 38 nt between tRNA-Asn and tRNA-Cys were detected during the annotation of this complete mitochondrial DNA.

摘要

使用下一代测序(NGS)方法确定了完整的有丝分裂基因组,并对本文中所得数据进行了分析。线粒体基因组长度为16493个碱基对(bp),其含量和结构与其他硬骨鱼类高度同源,包括13个蛋白质编码基因(PCG)、2个rRNA、22个tRNA和1个控制区。在PCG中,除COI基因中的GTG外,均以ATG作为起始密码子。共有6个重叠基因,重叠长度为1至10个核苷酸(nt),而在该完整线粒体DNA注释过程中检测到10个基因间隔区,总长度为59 nt,tRNA-Asn和tRNA-Cys之间的最大间隔为38 nt。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2e5/7721023/7fb81c5e717a/TMDN_A_1695550_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2e5/7721023/7fb81c5e717a/TMDN_A_1695550_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2e5/7721023/7fb81c5e717a/TMDN_A_1695550_F0001_B.jpg

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本文引用的文献

1
The complete mitochondrial genome sequence of the cutlassfish Trichiurus japonicus (Perciformes: Trichiuridae): Genome characterization and phylogenetic considerations.日本带鱼(鲈形目:带鱼科)线粒体全基因组序列:基因组特征及系统发育分析
Mar Genomics. 2009 Jun;2(2):133-42. doi: 10.1016/j.margen.2009.07.003. Epub 2009 Aug 26.
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RNA-Seq: a revolutionary tool for transcriptomics.RNA测序:转录组学的革命性工具。
Nat Rev Genet. 2009 Jan;10(1):57-63. doi: 10.1038/nrg2484.
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Molecular phylogeny of the medaka fishes genus Oryzias (Beloniformes: Adrianichthyidae) based on nuclear and mitochondrial DNA sequences.
基于核DNA和线粒体DNA序列的青鳉属(颌针鱼目:青鳉科)分子系统发育研究
Mol Phylogenet Evol. 2005 Aug;36(2):417-28. doi: 10.1016/j.ympev.2005.01.016. Epub 2005 Mar 5.
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The complete mitochondrial genome of the rockfish Sebastes schlegeli (Scorpaeniformes, Scorpaenidae).
Mol Cells. 2004 Apr 30;17(2):322-8.