Ma Jiangru, Long Shuisheng, Wang Zhongduo
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Mitochondrial DNA B Resour. 2019 Dec 9;5(1):27-28. doi: 10.1080/23802359.2019.1695550.
The complete mitogenome was determined using next-generation sequencing (NGS) method and the resulting data analyzed in this article. The mitochondrial genome was 16,493 base pair (bp) length, and its content and structure were highly homologous to that of other teleostean fishes, including 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNA, and 1 control region. Among the PCGs, ATG was used as the initiation codon, except for GTG in the COI gene. There were 6 overlapping genes with overlap lengths ranging from 1 to 10 nucleotides (nt), while 10 intergenic regions with a total of 59 nt and a maximum interval of 38 nt between tRNA-Asn and tRNA-Cys were detected during the annotation of this complete mitochondrial DNA.
使用下一代测序(NGS)方法确定了完整的有丝分裂基因组,并对本文中所得数据进行了分析。线粒体基因组长度为16493个碱基对(bp),其含量和结构与其他硬骨鱼类高度同源,包括13个蛋白质编码基因(PCG)、2个rRNA、22个tRNA和1个控制区。在PCG中,除COI基因中的GTG外,均以ATG作为起始密码子。共有6个重叠基因,重叠长度为1至10个核苷酸(nt),而在该完整线粒体DNA注释过程中检测到10个基因间隔区,总长度为59 nt,tRNA-Asn和tRNA-Cys之间的最大间隔为38 nt。