Kim Sang-Chul, Lee Jei-Wan, Lee Min-Woo, Baek Seung-Hoon, Hong Kyung-Nak
Division of Forest Genetic Resources, National Institute of Forest Science, Suwon, Republic of Korea.
Mitochondrial DNA B Resour. 2017 Dec 21;3(1):36-37. doi: 10.1080/23802359.2017.1419092.
The complete chloroplast (cp) genome sequence of and were determined by Ion torrent Platform sequencing in this study. The cp genome was 122,158bp consists of two inverted repeat (IR) regions of 436bp each, a large single-copy (LSC) region of 65,394bp, and a small single-copy (SSC) region of 55,892bp. The chloroplast genome sequence of var. was 122,573bp in length, consisting of two IRs (436bp), one LSC (65,597bp), and one SSC (56,104bp), and is longer than that of . The genome contained 110 genes, including 71 protein-coding genes, 35 tRNA genes, and 4 ribosomal RNA genes. The 13 genes contain introns, including 12 genes with a single intron each and gene with two introns. And, the rps12 gene is a trans-spliced gene. The phylogenetic analysis revealed that all sampled species in Pinaceae formed a monophyletic clade with high bootstrap value. The genus is closely related to .
在本研究中,通过离子激流平台测序确定了[物种名称1]和[物种名称2]的完整叶绿体(cp)基因组序列。[物种名称1]的cp基因组为122,158bp,由两个各为436bp的反向重复(IR)区域、一个65,394bp的大单拷贝(LSC)区域和一个55,892bp的小单拷贝(SSC)区域组成。[物种名称2]变种的叶绿体基因组序列长度为122,573bp,由两个IR(436bp)、一个LSC(65,597bp)和一个SSC(56,104bp)组成,比[物种名称1]的长。该基因组包含110个基因,包括71个蛋白质编码基因、35个tRNA基因和4个核糖体RNA基因。13个基因含有内含子,其中12个基因各有一个内含子,1个基因有两个内含子。并且,rps12基因是一个反式剪接基因。系统发育分析表明,松科所有采样物种形成了一个具有高自展值的单系类群。[属名1]属与[属名2]属密切相关。