Fang Min-Feng, Wang Yu-Jin, Zu Yu-Meng, Dong Wan-Lin, Wang Ruo-Nan, Deng Tuan-Tuan, Li Zhong-Hu
a Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education) , College of Life Sciences, Northwest University , Xi'an , China and.
b State Key Laboratory of Grassland Agro-Ecosystem , School of Life Sciences, Lanzhou University , Lanzhou , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2732-3. doi: 10.3109/19401736.2015.1046169. Epub 2015 Jun 9.
The complete nucleotide sequence of the Taiwan red pine Pinus taiwanensis Hayata chloroplast genome (cpDNA) is determined in this study. The genome is composed of 119,741 bp in length, containing a pair of very short inverted repeat (IRa and IRb) regions of 495 bp, which was divided by a large single-copy (LSC) region of 65,670 bp and a small single-copy (SSC) region of 53,080 bp in length. The cpDNA contained 115 genes, including 74 protein-coding genes (73 PCG species), 4 ribosomal RNA genes (four rRNA species) and 37 tRNA genes (22 tRNA species). Out of these genes, 12 harbored a single intron, and one (rps12) contained a couple of introns. The overall AT content of the Taiwan red pine cpDNA is 61.5%, while the corresponding values of the LSC, SSC and IR regions are 62.2%, 60.6% and 63.6%, respectively. A maximum parsimony phylogenetic analysis suggested that the genus Pinus, Picea, Abies and Larix were strongly supported as monophyletic, and the cpDNA of P. taiwanensis is closely related to that of P. thunbergii.
本研究测定了台湾红松(Pinus taiwanensis Hayata)叶绿体基因组(cpDNA)的完整核苷酸序列。该基因组长度为119,741 bp,包含一对长度为495 bp的非常短的反向重复(IRa和IRb)区域,被一个长度为65,670 bp的大单拷贝(LSC)区域和一个长度为53,080 bp的小单拷贝(SSC)区域隔开。cpDNA包含115个基因,包括74个蛋白质编码基因(73种PCG)、4个核糖体RNA基因(4种rRNA)和37个tRNA基因(22种tRNA)。在这些基因中,12个含有单个内含子,1个(rps12)含有几个内含子。台湾红松cpDNA的总体AT含量为61.5%,而LSC、SSC和IR区域的相应值分别为62.2%、60.6%和63.6%。最大简约法系统发育分析表明,松属、云杉属、冷杉属和落叶松属得到强烈支持为单系类群,台湾红松的cpDNA与黑松的cpDNA密切相关。