Jiang Jin-Qing, Wang Qiu-Xia, Liu Jun-Wei, Liu Chang-Zhong
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Mitochondrial DNA B Resour. 2018 Sep 27;3(2):1133-1134. doi: 10.1080/23802359.2018.1473732.
In this study, the complete mitochondrial genome of three-spined stickleback, was determined through sequencing of PCR fragments. The complete mitochondrial genome of was 16,543 bp in length and encoded 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and two ribosomal RNA genes. The overall nucleotide composition is: 27.0% A, 28.4% T, 27.4% C, and 17.2% G, with a total G + C content of 44.6%. By phylogenetic analysis using ML method, showed the closest relationship with the blackspotted stickleback ().
在本研究中,通过对PCR片段进行测序确定了三刺鱼的完整线粒体基因组。其完整线粒体基因组长度为16,543 bp,编码13个蛋白质编码基因、22个转移RNA(tRNA)基因和两个核糖体RNA基因。总体核苷酸组成为:A占27.0%,T占28.4%,C占27.4%,G占17.2%,G + C总含量为44.6%。通过使用最大似然法(ML)进行系统发育分析,结果表明其与黑点刺鱼()关系最为密切。