Milton A Arun Prince, Momin Kasanchi M, Ghatak Sandeep, Priya G Bhuvana, Angappan M, Das Samir, Puro K, Sanjukta R K, Shakuntala I, Sen A, Kandpal B K
Division of Animal Health, ICAR Research Complex for NEH Region, Umiam, Meghalaya, India.
College of Agriculture, Central Agricultural University (Imphal), Kyrdemkulai, Meghalaya, India.
Heliyon. 2021 Jan 12;7(1):e05941. doi: 10.1016/j.heliyon.2021.e05941. eCollection 2021 Jan.
is a widespread foodborne pathogen and one of the major concerns in the meat industry. There is a need for a simple, rapid and equipment free detection system for as conventional anaerobic culture method is labour and resource intensive. Here, we applied a novel polymerase spiral reaction phenomenon to develop and evaluate an assay for effortless and visual detection of in meat foods employing pork as a representative model. Specificity of the assay was determined using 51 and 20 non- strains. Analytical sensitivity of the developed test was 80 fg DNA per tube indicating 100 times more sensitivity than end-point PCR assay. The detection limits were 980 CFU/g and 9.8 × 10 CFU/g of pork for PSR and PCR assays, respectively. The operation time of the PSR assay including DNA extraction was 120 min. The developed PSR assay was accurate and effective in comparison to culture method, in detecting in 38 of 74 pork samples. Therefore the specificity, sensitivity, negative predictive value, positive predictive value and accuracy rate of the developed PSR assay were 100%. The developed PSR assay is easy to perform, rapid, affordable, permitting sophisticated-equipment free amplification and naked eye interpretation. This is the initial report in which the PSR assay was optimized for the detection of
是一种广泛存在的食源性病原体，也是肉类行业的主要关注点之一。由于传统的厌氧培养方法耗费人力和资源，因此需要一种简单、快速且无需设备的检测系统。在此，我们应用了一种新型的聚合酶螺旋反应现象，以猪肉为代表性模型，开发并评估了一种用于轻松直观检测肉类食品中 的检测方法。使用51株 和20株非 菌株确定了该检测方法的特异性。所开发检测方法的分析灵敏度为每管80 fg DNA，表明其灵敏度比终点PCR检测方法高100倍。PSR和PCR检测方法对猪肉的检测限分别为980 CFU/g和9.8×10 CFU/g。包括DNA提取在内的PSR检测方法的操作时间为120分钟。与培养方法相比，所开发的PSR检测方法在检测74份猪肉样品中的38份时准确有效。因此，所开发的PSR检测方法的特异性、灵敏度、阴性预测值、阳性预测值和准确率均为100%。所开发的PSR检测方法易于操作、快速、经济实惠，无需复杂设备即可进行扩增并可通过肉眼解读。这是首次针对检测 对PSR检测方法进行优化的报告
