Usui T, Hayashi Y, Nanjo F, Ishido Y
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Biochim Biophys Acta. 1988 Mar 23;953(2):179-84.
Transchitooligosylation from (GlcNAc)5 to the 4-position of PNP-GlcNAc was efficiently induced through lysozyme catalysis in an aqueous solution containing methanol with a high concentration. Use of the aqueous methanol system in this reaction not only guaranteed solubility of PNP-GlcNAc substrate, but also resulted in a remarkable increase in PNP-(GlcNAc)5 production. PNP-(GlcNAc)5 was substrate for lysozyme assay compared with PNP-(GlcNAc)4.
在含有高浓度甲醇的水溶液中，通过溶菌酶催化可有效地诱导从（GlcNAc）5到PNP-GlcNAc 4位的转壳寡糖基化反应。在该反应中使用甲醇水溶液体系不仅保证了PNP-GlcNAc底物的溶解性，而且还使PNP-（GlcNAc）5的产量显著增加。与PNP-（GlcNAc）4相比，PNP-（GlcNAc）5是溶菌酶测定的底物。
