European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DW, UK.
STAR Protoc. 2021 Jan 13;2(1):100253. doi: 10.1016/j.xpro.2020.100253. eCollection 2021 Mar 19.
This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020).
本方案说明了将冷冻结构光照显微镜和冷冻软 X 射线断层扫描的相关 3D 冷冻成像数据存入欧洲生物信息研究所的 BioStudies 和 EMPIAR 数据库所需的步骤。目前，非常需要一种可靠的数据存储方法，以确保能够无障碍地访问和独立验证相关的光镜和 X 射线显微镜数据，从而可以进一步用于比较研究、教育活动和数据挖掘。有关本方案的使用和执行的详细信息，请参考 Kounatidis 等人（2020 年）。
