大鼠有髓神经郎飞结箝压式膜片钳记录的实验方案。

Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Department of Pharmacology, Hyogo University of Health Sciences, Kobe, Hyogo 650-8530, Japan.

STAR Protoc. 2021 Jan 12;2(1):100266. doi: 10.1016/j.xpro.2020.100266. eCollection 2021 Mar 19.

The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals. For complete information on the generation and use of this protocol, please refer to Kanda et al. (2019).

膜片钳记录技术对于研究细胞离子通道功能是不可或缺的，但将其应用于郎飞结（Ranvier node）具有挑战性，郎飞结是沿有髓神经传导动作电位的关键部位。我们已经开发了一种应用于大鼠有髓神经郎飞结的压力钳膜片钳记录方法。本文描述的逐步方案使研究人员能够应用这种方法来研究哺乳动物有髓神经中的跳跃传导和信息处理的机制。有关此方案的生成和使用的完整信息，请参考 Kanda 等人（2019 年）。