Department of Biology, Duke University, Durham, NC 27708, USA.
Center for Genomic and Computational Biology, Duke University, Durham, NC 27708, USA.
STAR Protoc. 2021 Jan 14;2(1):100276. doi: 10.1016/j.xpro.2020.100276. eCollection 2021 Mar 19.
Standard laboratory culture of utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).
标准的实验室培养利用固体生长培养基和细菌食物源。然而，这种培养方法限制了食物供应和虫体密度的控制，而这些因素会影响许多生活史特征。在这里，我们描述了液体培养方案，可以精确调节细菌食物供应和种群密度，从而可靠地生产出被阻止的 L1 幼虫、 dauer 幼虫、饮食受限的蠕虫或饱食的蠕虫。可以将蠕虫少量培养用于标准测定，也可以大量培养用于其他应用。有关这些方案的使用和实施的完整详细信息，请参考 Hibshman 等人（2016 年）、Webster 等人（2018 年）和 Jordan 等人（2019 年）。
