细菌视紫红质的K和KL中间体的时间分辨光谱研究。
A time-resolved spectral study of the K and KL intermediates of bacteriorhodopsin.
作者信息
Milder S J, Kliger D S
机构信息
Department of Chemistry, University of California, Santa Cruz 95064.
出版信息
Biophys J. 1988 Mar;53(3):465-8. doi: 10.1016/S0006-3495(88)83124-2.
Abstract
Nanosecond time-resolved absorption measurements on the photolysis products of bacteriorhodopsin (BR) in intact membranes are reported. At room temperature in fluid solution a single intermediate (KL) is seen 10 ns after excitation. Both spectral and kinetic results are consistent with the KL intermediate converting to the L intermediate by a single first order reaction. The observed temperature-dependent rate has the Arrhenius parameters: Ea = 10.5 kcal/mol, A = 5 x 10(13) s-1. The precursor to the KL intermediate is also observed. Its spectral character is consistent with the K intermediate which has been previously reported. The current data is consistent with a linear sequence in the BR photocycle for K, KL, and L in room temperature fluid solution. Differences in the spectral characteristics of the K intermediates described here and elsewhere are discussed in terms of differences in the microenvironment around the retinal moiety and the affect this may have on the conformation of the chromophore.
摘要
报道了对完整膜中细菌视紫红质（BR）光解产物的纳秒时间分辨吸收测量结果。在室温下的流体溶液中，激发后10纳秒可见单一中间体（KL）。光谱和动力学结果均表明，KL中间体通过单一的一级反应转化为L中间体。观察到的温度依赖性速率具有阿伦尼乌斯参数：活化能Ea = 10.5千卡/摩尔，频率因子A = 5×10¹³ 秒⁻¹。还观察到了KL中间体的前体。其光谱特征与先前报道的K中间体一致。目前的数据与室温流体溶液中BR光循环中K、KL和L的线性序列一致。本文所述及其他地方描述的K中间体光谱特征差异，根据视黄醛部分周围微环境的差异以及这可能对发色团构象产生的影响进行了讨论。
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