Lazarevich E V, Samoĭlov M O, Semenov D G
Biull Eksp Biol Med. 1988 Mar;105(3):261-4.
Changes in membrane-bound calcium (Ca2+(b)) content in the brain cortex membrane structures were studied on subcellular fractions (synaptosomes, microsomes, mitochondria) during in vitro anoxia. The changes in Ca2+ content in hydrophobic domains of intracellular membranes were assessed, using chlorotetracycline fluorescent probe. It has been found that membranes of different neuronal compartments are not equally vulnerable to anoxia. A decrease in Ca2+9(b) content in response to anoxia occurs in synaptosomes and microsomes much sooner than in mitochondria. Therefore, Ca2+ release from intracellular membrane compartments, preceding the massive inward flow of extracellular Ca2+, seems to be one of those mechanisms initiating a complex range of intracellular reactions to disturbed oxygen supply in brain cortex neurons.
在体外缺氧期间，研究了大脑皮质膜结构中膜结合钙（Ca2+(b)）含量在亚细胞组分（突触体、微粒体、线粒体）中的变化。使用氯四环素荧光探针评估细胞内膜疏水结构域中Ca2+含量的变化。结果发现，不同神经元区室的膜对缺氧的敏感性不同。突触体和微粒体中，缺氧导致的Ca2+9(b)含量降低比线粒体中早得多。因此，在细胞外Ca2+大量内流之前，细胞内膜区室释放Ca2+似乎是引发大脑皮质神经元对氧供应紊乱产生一系列复杂细胞内反应的机制之一。
