College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China.
Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease by Henan & Education Ministry of PR China, Zhengzhou, China.
J Sci Food Agric. 2021 Aug 30;101(11):4672-4680. doi: 10.1002/jsfa.11112. Epub 2021 Feb 17.
Purple corn (Zea mays L.) is one of the main economic crops in China and has been used in the treatment of cystitis, urinary infections and obesity. However, purple corncobs, the by-product remaining after processing and having an intense purple-black color, are normally disposed of as waste or used as animal feed. Therefore, to further expand the medicinal value of purple corncob, its content was analyzed and, after purification, the effect and mechanism of purified purple corncob anthocyanins (PPCCA) on CCl -induced chronic liver injury in mice were investigated.
It was observed that the total anthocyanin content (TAC) from PPCCA (317.51 ± 9.30 mg cyanidin 3-O-glucoside (C-3-G) g dry weight) was significantly higher than that from the purified purple corn seed anthocyanin (266.73 ± 3.67 mg C-3-G g dry weight), of which C-3-G accounted for 90.6% and 90.4% of the TAC, respectively. Furthermore, compared with the CCl group, PPCCA treatment significantly reduced liver index, serum total bilirubin, alanine transaminase, aspartate transaminase and liver malondialdehyde levels, but increased liver superoxide dismutase activity. The pathological changes were also improved, such as more regular arrangement of hepatocytes, less swelling, and fewer vacuoles and apoptotic cells. Additionally, mechanistic studies showed that PPCCA downregulated the expression of Caspase-3, Bax and cytochrome P450 2E1 proteins in the liver and upregulated the expression of Bcl-2.
These results demonstrated that PPCCA could ameliorate CCl -induced chronic liver injury by regulating oxidative stress and hepatocyte apoptosis pathways. © 2021 Society of Chemical Industry.
紫玉米（Zea mays L.）是中国的主要经济作物之一，已用于治疗膀胱炎、尿路感染和肥胖症。然而，紫玉米芯是加工后的副产物，颜色深紫黑，通常被当作废物丢弃或用作动物饲料。因此，为了进一步扩大紫玉米芯的药用价值，分析了其含量，经纯化后，研究了纯化的紫玉米芯花色苷（PPCCA）对 CCl 诱导的慢性肝损伤小鼠的作用及机制。
观察到 PPCCA 的总花色苷含量（TAC）（317.51±9.30mg 矢车菊素 3-O-葡萄糖苷（C-3-G）/g 干重）显著高于纯化的紫玉米种子花色苷（266.73±3.67mg C-3-G/g 干重），其中 C-3-G 分别占 TAC 的 90.6%和 90.4%。此外，与 CCl 组相比，PPCCA 处理显著降低了肝指数、血清总胆红素、丙氨酸转氨酶、天冬氨酸转氨酶和肝丙二醛水平，但增加了肝超氧化物歧化酶活性。病理变化也得到改善，如肝细胞排列更规则，肿胀减轻，空泡和凋亡细胞减少。此外，机制研究表明，PPCCA 下调了肝脏中 Caspase-3、Bax 和细胞色素 P450 2E1 蛋白的表达，上调了 Bcl-2 的表达。
这些结果表明，PPCCA 可以通过调节氧化应激和肝细胞凋亡途径改善 CCl 诱导的慢性肝损伤。© 2021 英国化学学会。
