Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.
Oncology Institute of Vojvodina, Put dr Goldmana 4, Sremska Kamenica, Serbia.
Arch Pharm (Weinheim). 2021 May;354(5):e2000416. doi: 10.1002/ardp.202000416. Epub 2021 Jan 25.
Pinus nigra Arn. bark extracts from Mokra gora (MG) and Tara mountains were analyzed with regard to their polyphenolic profile and antioxidative and antiproliferative activity. The ethanol extract from MG showed the highest phenolic, flavonoid, tannin, and proanthocyanidin content when compared with the acetone and methanol extracts from both sites. The same extract exhibited the highest ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) and ferric reducing antioxidant power (FRAP) radical scavenging ability and total antioxidant activity (TAA). On the basis of high-performance liquid chromatography analysis, catechin, caffeic, syringic, p-coumaric, and ferulic acids were predominantly present in the MG extracts. The ethanol extract from MG was rich in syringic acid, epicatechin and its derivatives, and ferulic acid and its derivatives. The bark extracts also exerted a high cytotoxic bioactivity against all evaluated cell lines (HeLa, MCF7, HT-29, and MRC-5). The antiproliferative activity of P. nigra bark is probably related to the ferulic acid content and its synergistic activity to caffeic acid and taxifolin. The antioxidative role of the presented phenols was confirmed through the obtained significant linear correlation between the total phenolic content and DPPH (r = .934) as well as the FRAP% of the extracts (r = .948). Also, the TAA significantly depended on the proanthocyanidins (r = .902) and tannin contents (r = .914). The composition of the presented compounds could be related to promising antioxidant and antiproliferative efficacy of MG bark.
黑松（Pinus nigra Arn.）摩拉哥拉（Mokra gora，MG）和塔拉山的树皮提取物，就其多酚谱和抗氧化及抗增殖活性进行了分析。与来自这两个地点的丙酮和甲醇提取物相比，MG 的乙醇提取物表现出最高的酚类、类黄酮、单宁和原花青素含量。该提取物还表现出最高的 ABTS（2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐）和铁还原抗氧化能力（FRAP）自由基清除能力和总抗氧化活性（TAA）。基于高效液相色谱分析，MG 提取物中主要存在儿茶素、咖啡酸、丁香酸、对香豆酸和阿魏酸。MG 乙醇提取物富含丁香酸、表儿茶素及其衍生物以及阿魏酸及其衍生物。树皮提取物对所有评估的细胞系（HeLa、MCF7、HT-29 和 MRC-5）均表现出很高的细胞毒性生物活性。黑松树皮的抗增殖活性可能与阿魏酸含量及其与咖啡酸和 taxifolin 的协同活性有关。所获得的 DPPH（r =.934）和提取物 FRAP%（r =.948）之间的总酚含量与抗氧化活性之间的显著线性相关性证实了所提出的酚类化合物的抗氧化作用。此外，TAA 还显著依赖于原花青素（r =.902）和单宁含量（r =.914）。所提出化合物的组成可能与 MG 树皮的有希望的抗氧化和抗增殖功效有关。
