评价药用珍贵蕨类植物 coadunata 的抗癌、抗菌和抗氧化特性及其通过 HR-LCMS 分析的植物成分。

Evaluation of Anticancer, Antibacterial and Antioxidant Properties of a Medicinally Treasured Fern Tectaria coadunata with its Phytoconstituents Analysis by HR-LCMS.

机构信息

Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat, Gujarat, India.

Department of Biology, College of Science, University of Ha'il, Ha'il, PO Box 2440, Saudi Arabia.

出版信息

Anticancer Agents Med Chem. 2020;20(15):1845-1856. doi: 10.2174/1871520620666200318101938.

DOI:10.2174/1871520620666200318101938

PMID:32188388

Abstract

BACKGROUND

Tectaria coadunata (T. coadunata) is an important fern species with a number of medicinal properties. It has been evidently found for its effectiveness in ethanomedicinal usage, which can also emerge as one of the most promising sources for nutraceuticals.

OBJECTIVE

This study aims to examine the phytochemistry of the whole crude extract of T. coadunata for the first time with evaluation of antibacterial, antioxidant and anticancer activity.

METHODS

High Resolution Liquid Chromatography Mass Spectrometry analysis (HR-LCMS) was performed for confirming the presence of biologically active constituents in the extract of T. coadunata followed by antibacterial, antioxidant and anticancer activity.

RESULTS

With the detailed Mass spectra data, absorbance spectra and retention times, chemical composition of T. coadunata holds a diverse group of bioactive/chemical components such as sugars, sugar alcohol, flavonoids, terpenoids and phenolics. The results for antioxidant activity showed that T. coadunata crude extract had higher scavenging potential against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals than H2O2 molecules, which was followed by positive antibacterial activity against several pathogenic bacteria like Shigella flexneri, Staphylococcus aureus and Salmonella typhi.

DISCUSSION

The ethanolic extract of T. coadunata showed favorable antiproliferation activity against three leukemic (KG1, MOLT-3 and K-562) cells in a dose dependent manner, especially for KG1 42.850±1.24μg/ml.

CONCLUSION

This study has provided a better understanding of the presence of biologically active phytochemical constituents in the extract of T. coadunata, which can be the reason for its bioactive potential. Moreover, T. coadunata has significant anticancer activities against human leukemic cancer cell lines, indicating it as a potential anticancer agent.

摘要

背景

蜈蚣草（T. coadunata）是一种具有多种药用特性的重要蕨类植物。它在乙醇药用方面的功效已被明显证实，也可能成为营养保健品最有前途的来源之一。

目的

本研究首次对蜈蚣草全粗提取物的植物化学进行研究，并评价其抗菌、抗氧化和抗癌活性。

方法

采用高分辨液相色谱-质谱联用技术（HR-LCMS）对蜈蚣草提取物中存在的生物活性成分进行分析，然后进行抗菌、抗氧化和抗癌活性评价。

结果

根据详细的质谱数据、吸光度谱和保留时间，蜈蚣草的化学成分包含了多种生物活性/化学成分，如糖、糖醇、类黄酮、萜类化合物和酚类化合物。抗氧化活性结果表明，蜈蚣草粗提取物对 2,2-二苯基-1-苦基肼（DPPH）自由基的清除能力高于对 H2O2 分子的清除能力，并且对志贺氏菌属、金黄色葡萄球菌和伤寒沙门氏菌等几种致病菌具有阳性抗菌活性。