可乐定和吗啡可增加小鼠输精管中[3H]-去甲肾上腺素的溢出。

Clonidine and morphine increase [3H]-noradrenaline overflow in mouse vas deferens.

作者信息

Forsyth K M, Pollock D

机构信息

Department of Pharmacology, University of Glasgow.

出版信息

Br J Pharmacol. 1988 Jan;93(1):35-42. doi: 10.1111/j.1476-5381.1988.tb11402.x.

DOI:10.1111/j.1476-5381.1988.tb11402.x

PMID:3349232

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1853765/

Abstract

Field stimulation of mouse isolated vas deferens produced a biphasic contraction that consisted of an initial brief non-adrenergic, non-cholinergic (NANC) twitch, followed by a more prolonged noradrenergic component. 2. Field stimulation of vasa, previously loaded with [3H]-noradrenaline ([3H]-NA), increased the amount of radioactivity in the Krebs bathing solution; 77% of this radioactivity was derived from [3H]-NA. 3. Tetrodotoxin (3 x 10(-6) M) abolished the biphasic motor response to field stimulation and the accompanying increased overflow of [3H]-NA. 4. Morphine (10(-7)-10(-5) M) inhibited the initial NANC component but potentiated the secondary noradrenergic component of the motor response to field stimulation. Morphine also increased the field stimulation-induced overflow of radioactivity. Naloxone (10(-6) M) antagonized the effects of morphine on the motor response and also on the overflow of radioactivity. 5. Clonidine (10(-9)-10(-7) M) inhibited the initial NANC component but potentiated the secondary noradrenergic component of the motor response to field stimulation. Clonidine also increased the field stimulation-induced overflow of radioactivity. 6. The ability of morphine (10(-7) M) and of clonidine (10(-9) M) to potentiate the field stimulation-induced overflow of radioactivity persisted in the presence of a combination of tranylcypromine (10(-5) M), desmethylimipramine (10(-5) M) and 17-beta-oestradiol (10(-5) M). 7. The inhibition of the initial NANC component of the motor response to field stimulation produced by morphine and clonidine may be related to the ability of these drugs to potentiate both the secondary noradrenergic component and the overflow of radioactivity, if the NANC transmitter is involved in regulating NA release.

摘要

对小鼠离体输精管进行场刺激会产生双相收缩，其包括最初短暂的非肾上腺素能、非胆碱能（NANC）抽搐，随后是持续时间更长的去甲肾上腺素能成分。2. 对预先加载了[3H] - 去甲肾上腺素（[3H] - NA）的输精管进行场刺激，会增加 Krebs 浴液中的放射性；这种放射性的 77%来自[3H] - NA。3. 河豚毒素（3×10⁻⁶ M）消除了对场刺激的双相运动反应以及伴随的[3H] - NA 溢出增加。4. 吗啡（10⁻⁷ - 10⁻⁵ M）抑制最初的 NANC 成分，但增强了对场刺激的运动反应的继发性去甲肾上腺素能成分。吗啡还增加了场刺激诱导的放射性溢出。纳洛酮（10⁻⁶ M）拮抗吗啡对运动反应以及放射性溢出的影响。5. 可乐定（10⁻⁹ - 10⁻⁷ M）抑制最初的 NANC 成分，但增强了对场刺激的运动反应的继发性去甲肾上腺素能成分。可乐定还增加了场刺激诱导的放射性溢出。6. 在存在反苯环丙胺（10⁻⁵ M）、去甲丙咪嗪（10⁻⁵ M）和 17 - β - 雌二醇（10⁻⁵ M）的组合时，吗啡（10⁻⁷ M）和可乐定（10⁻⁹ M）增强场刺激诱导的放射性溢出的能力仍然存在。7. 如果 NANC 递质参与调节 NA 释放，那么吗啡和可乐定对场刺激的运动反应的最初 NANC 成分的抑制可能与这些药物增强继发性去甲肾上腺素能成分和放射性溢出的能力有关。