Department of Plastic, Reconstructive and Aesthetic Surgery, Abant Izzet Baysal University, Gölköy, Bolu, Turkey.
Aesthet Surg J. 2021 Jun 14;41(7):NP887-NP901. doi: 10.1093/asj/sjab043.
The clinical results of many studies on platelet-rich plasma (PRP) differ due to a lack of standardization in PRP preparation and administration as well as many variables such as PRP preparation methods, platelet concentration, and platelet activation.
The authors sought to investigate a different variable that will affect PRP application results. How much PRP should be injected into the unit area of tissue for an effective PRP treatment?
The study was performed on fresh surplus tissues of 20 patients that were discarded in abdominoplasty and mammoplasty operations. Nine areas of 4 cm2 were marked on the skin. Fluorescein-stained PRP was injected intradermally with 3 different gauge needles at 3 different doses (0.01, 0.03, and 0.05 mL). After injections, spreads of the fluorescent dye-covered areas in horizontal and vertical planes were measured and compared. For the horizontal plane measurements, the dye spread was measured first from the surface of the skin and second from the dermal surface of the skin. In addition, the width and depth of the dye spread in the dermis were measured from vertical sections.
Changing the needle diameter does not affect the width or depth (thickness) of the PRP spread in the dermis. Increasing the applied dose to 0.03 mL increases the spread to the width and depth (thickness).
In research evaluating the effectiveness of PRP treatments, it is necessary to report the volume of PRP to be applied per unit of tissue.
由于缺乏 PRP 制备和管理的标准化，以及 PRP 制备方法、血小板浓度和血小板激活等许多变量，许多关于富血小板血浆（PRP）的研究的临床结果存在差异。
作者旨在研究一个会影响 PRP 应用结果的变量。为了进行有效的 PRP 治疗，应该向单位面积的组织中注射多少 PRP？
该研究在腹部整形术和乳房成形术中丢弃的 20 名患者的新鲜剩余组织上进行。在皮肤上标记 9 个 4cm2 的区域。用 3 种不同规格的针头以 3 种不同剂量（0.01、0.03 和 0.05mL）将荧光素染色的 PRP 皮内注射。注射后，测量并比较水平和垂直平面上荧光染料覆盖区域的扩散情况。对于水平平面测量，首先从皮肤表面测量染料的扩散，然后从皮肤真皮表面测量。此外，还从垂直切片测量真皮中染料扩散的宽度和深度。
改变针头直径不会影响 PRP 在真皮中的扩散宽度或深度（厚度）。将施加的剂量增加到 0.03mL 会增加宽度和深度（厚度）的扩散。
在评估 PRP 治疗效果的研究中，有必要报告每单位组织应用的 PRP 体积。
