在当地库蚊（双翅目：蚊科）中，阿根廷西尼罗河病毒株的易感性和传播性的剂量依赖性。
Dose dependence of susceptibility and transmission for an Argentinean West Nile virus strain in local Culex pipiens quinquefasciatus (Diptera: Culicidae).
机构信息
Instituto de Investigaciones Biológicas y Tecnológicas - IIBYT, CONICET - Universidad Nacional de Córdoba, Córdoba (X5016GCA), Argentina.
Laboratorio de Arbovirus, Instituto de Virología "Dr J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba (CP5016), Argentina.
出版信息
Trans R Soc Trop Med Hyg. 2021 Sep 3;115(9):1066-1069. doi: 10.1093/trstmh/traa185.
BACKGROUND
West Nile virus (WNV) activity has been documented in the central region of Argentina since 2005, but its maintenance network has not been elucidated yet.
METHODS
We evaluated the susceptibility to WNV oral infection and transmission in a Culex pipiens quinquefasciatus mosquito population by means of a dose-dependent vector competence assay. Mosquitoes were orally infected with five different viral loads and evaluated for viral infection, dissemination and transmission.
RESULTS
The evaluated population was susceptible to WNV oral infection. Disseminated infections were detected in all the viral doses but transmission was only detected in the higher doses (6 and 7 log10 plaque-forming units/ml). A linear relationship between doses and transmission rates could be established.
CONCLUSIONS
A minimum transmission threshold suggests that the Cu. p. quinquefasciatus from Argentina could acquire WNV infection from local urban birds and transmit the virus.
背景
自 2005 年以来，阿根廷中部地区已有西尼罗河病毒（WNV）活动的记录，但尚未阐明其维持网络。
方法
我们通过剂量依赖性媒介效能测定评估了携带五重条纹库蚊种群对 WNV 口服感染和传播的易感性。用五种不同病毒载量对蚊子进行口服感染，并评估病毒感染、传播和传播情况。
结果
评估的种群易受 WNV 口服感染。在所有病毒剂量中均检测到传播感染，但仅在较高剂量（6 和 7 log10 噬菌斑形成单位/ml）中检测到传播。可以建立剂量与传播率之间的线性关系。
结论
最低传播阈值表明，来自阿根廷的 Cu. p. quinquefasciatus 可能从当地城市鸟类中获得 WNV 感染并传播该病毒。
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