Immunology laboratory, University Hospital Saint-Etienne, 42055 Saint-Etienne, Cedex 2, France.
Allergology unit, University Hospital Saint-Etienne, 42055 Saint-Etienne, Cedex 2, France.
J Immunol Methods. 2021 May;492:112951. doi: 10.1016/j.jim.2020.112951. Epub 2021 Jan 22.
Basophils play a major physio-pathological role in hypersensitivity related diseases. Basophils express high affinity Immunoglobulin (Ig) E receptors (FcεRI), IgG and complement regulatory. Basophils also have immunoregulatory activity through interaction with T cells. The aim of this study was to look for the expression of markers reflecting the activation status of peripheral Basophil in healthy donors.
the study was performed on 29 healthy donors, 62% females with a mean age of 50.1 + 17.0 years. Basophils were identified on their expression of CD123 without HLA-DR and/or CD193 in two 8 colors panels including CD46, CD55, CD59, CD203c, CD32 (FcγRII), CD64 (FcγRIII), CD163, CD137L (4-1BBL), CD252 (OX40L), CD244 (2B4) and CD3 on whole blood. Basophil activation with anti IgE was performed on 14 donors.
Our results confirmed the Basophil expression of CD123, CD193 and CD203 (the latter is strongly increased under stimulation). Complement regulatory proteins (CD46, CD55, CD59) were expressed at the same levels as on other leukocytes; CD46, CD59 expression being slightly increased under stimulation. CD32 and CD163 scavenger were slightly higher than on lympho and not influenced by activation. CD252 or CD137L were expressed at low levels and significantly induced by stimulation. Most of all, CD244 was highly expressed on Basophils as compared to any other leukocytes in fresh peripheral blood.
Our study shows that human resting Basophils express IgE and IgG Fc receptors and check point receptor CD244 that could potentially play a role in their previously reported immunoregulatory activity in sensitization and even in tumor immune escape.
嗜碱性粒细胞在与过敏相关的疾病中起着重要的生理病理作用。嗜碱性粒细胞表达高亲和力免疫球蛋白（Ig）E 受体（FcεRI）、IgG 和补体调节蛋白。嗜碱性粒细胞还通过与 T 细胞相互作用具有免疫调节活性。本研究旨在寻找反映健康供者外周嗜碱性粒细胞激活状态的标志物。
本研究共纳入 29 名健康供者，其中女性占 62%，平均年龄为 50.1±17.0 岁。通过流式细胞术在两个 8 色面板中检测嗜碱性粒细胞的 CD123 表达，该面板不包括 HLA-DR 和/或 CD193，同时检测 CD46、CD55、CD59、CD203c、CD32（FcγRII）、CD64（FcγRIII）、CD163、CD137L（4-1BBL）、CD252（OX40L）、CD244（2B4）和 CD3 的表达。对 14 名供者进行抗 IgE 刺激的嗜碱性粒细胞激活实验。
本研究结果证实了嗜碱性粒细胞表达 CD123、CD193 和 CD203（后者在刺激下强烈增加）。补体调节蛋白（CD46、CD55、CD59）在白细胞上的表达水平相同；刺激下 CD46、CD59 的表达略有增加。CD32 和 CD163 清除受体略高于淋巴细胞，不受激活影响。CD252 或 CD137L 表达水平较低，刺激后显著增加。最重要的是，CD244 在新鲜外周血中的嗜碱性粒细胞上表达水平明显高于任何其他白细胞。
本研究表明，人类静止嗜碱性粒细胞表达 IgE 和 IgG Fc 受体以及检查点受体 CD244，这可能在其先前报道的在致敏过程中的免疫调节活性中发挥作用，甚至在肿瘤免疫逃逸中发挥作用。
