National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, Henan, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng, 475004, China.
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, Henan, China.
Food Chem Toxicol. 2021 Mar;149:112010. doi: 10.1016/j.fct.2021.112010. Epub 2021 Jan 22.
In this paper, the Caco-2 cell was used to study the glucose absorption regulation and mechanism of kaempferol, caffeic acid and quercetin-3-O-β-D-galactoside in Lilium lancifolium Thunb in vitro. Glucose oxidase-peroxidase (GOD-POD) method was used to measure glucose consumption in supernatant. The fluorescent D-glucose analog (2-NBDG) was used as a tracer probe to study the changes in the fluorescence intensity of 2-NBDG uptake by Caco-2 cells with an inverted fluorescence microscope. Western blotting and quantitative real-time PCR were used to detect the protein expression and mRNA transcription of SGLT1 and GLUT2. The results showed that caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote the absorption of glucose by normal Caco-2 cells compared with the control group (P < 0.001). Both caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote the uptake of glucose tracer 2-NBDG on Caco-2 cells. Caffeic acid and quercetin-3-O-β-D-galactoside could significantly promote SGLT1 and GLUT2 protein expression levels and mRNA transcription (P < 0.001, P < 0.01, P < 0.05). The mechanism might be related to the promotion of SGLT1 and GLUT2 protein expression levels and mRNA transcription.
本文以百合鳞茎为研究对象，采用 Caco-2 细胞模型，体外研究山奈酚、咖啡酸和毛蕊花糖苷对葡萄糖吸收的调控作用及其机制。采用葡萄糖氧化酶-过氧化物酶（GOD-POD）法检测上清液中葡萄糖的消耗量，荧光标记的 2-NBDG 作为示踪探针，通过倒置荧光显微镜观察 Caco-2 细胞摄取 2-NBDG 的荧光强度变化，Western blot 和实时荧光定量 PCR 检测 SGLT1 和 GLUT2 蛋白表达和 mRNA 转录水平。结果表明，与对照组相比，咖啡酸和毛蕊花糖苷能显著促进正常 Caco-2 细胞对葡萄糖的吸收（P<0.001）。咖啡酸和毛蕊花糖苷均能显著促进 Caco-2 细胞摄取葡萄糖示踪剂 2-NBDG。咖啡酸和毛蕊花糖苷能显著促进 SGLT1 和 GLUT2 蛋白表达水平和 mRNA 转录（P<0.001，P<0.01，P<0.05）。其机制可能与促进 SGLT1 和 GLUT2 蛋白表达水平和 mRNA 转录有关。
