Wei Jinfeng, Wang Baoguang, Chen Yixiao, Wang Qiuyi, Ahmed Adel F, Zhang Yan, Kang Wenyi
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China.
Shenzhen Research Institute of Henan University, Shenzhen, China.
Front Nutr. 2022 May 31;9:899797. doi: 10.3389/fnut.2022.899797. eCollection 2022.
is a valuable herb for its functional compositions in both food and medication. seeds can enhance immunity, anti-inflammation and analgesia and hypoglycemia, but most of the related researches are related to volatile oil and extracts, and the activity and mechanism of compounds is not clear. In this study, Ethyl-α-D-galactopyranoside (EG), Methyl-α-D-glucoside (MG), 3--[β--xylopyranose-(1 → 3)-α--rhamnose-(1 → 2)-α--arabinose]-28--[α--rhamnose-(1 → 4)-β--glucopyranose--(1 → 6)-β--glucopyranose]-hederagenin (HXRARG) and 3-[β--xylopyranose-(1 → 3)-α--rhamnose-(1 → 2)-α--arabinose]-hederagenin (HXRA) were isolated and identified from seeds. In addition, four compounds could activate NF-κB pathway by promoting the expression of phosphorylation of P65 and IκBα, promoting the phosphorylation of JNK, Erk and P38 to activate MAPK signaling pathway, enhancing the proliferation and phagocytic activity of RAW264.7 cells, and promoting the release of NO, TNF-α and IL-6 on RAW264.7 cell . The results showed that can be used as dietary supplement to enhance immune.
因其在食品和药物中的功能成分而成为一种珍贵的草药。其种子可增强免疫力、抗炎、镇痛和降血糖，但大多数相关研究都与挥发油和提取物有关，化合物的活性和作用机制尚不清楚。在本研究中，从其种子中分离并鉴定出α-D-吡喃半乳糖乙酯（EG）、α-D-葡萄糖苷甲酯（MG）、3-[[β-D-吡喃木糖-（1→3）-α-L-鼠李糖-（1→2）-α-L-阿拉伯糖]-28-[[α-L-鼠李糖-（1→4）-β-D-吡喃葡萄糖-（1→6）-β-D-吡喃葡萄糖]-常春藤皂苷元（HXRARG）和3-[[β-D-吡喃木糖-（1→3）-α-L-鼠李糖-（1→2）-α-L-阿拉伯糖]-常春藤皂苷元（HXRA）。此外，四种化合物可通过促进P65和IκBα磷酸化的表达来激活NF-κB通路，促进JNK、Erk和P38的磷酸化以激活MAPK信号通路，增强RAW264.7细胞的增殖和吞噬活性，并促进RAW264.7细胞释放NO、TNF-α和IL-6。结果表明，其可作为膳食补充剂来增强免疫力。
