Breeding Informatics Group, Department of Animal Sciences, Georg-August University, Margarethe von Wrangell-Weg 7, 37075 Göttingen, Germany.
College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
Int J Mol Sci. 2021 Jan 21;22(3):1033. doi: 10.3390/ijms22031033.
Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF-TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.
转录因子（TFs）及其复杂的相互作用对于指导特定的遗传程序至关重要，例如通过调节基因表达对环境压力、组织发育或细胞分化的反应。了解 TF-TF 合作关系可能有助于深入了解 L. 品种之间的发育开关，即中双 11（ZS11），一种高油含量的双低品种，和中油 821（ZY821），一种低油含量的双高品种。在这方面，我们主要关注单调表达基因（MEGs），对来自这两个品种的种子组织进行了时间序列 RNA-seq 数据集分析。考虑 MEGs 可以捕获由协同 TF 协调的多阶段进展过程，从而有助于理解决定种子油含量的分子机制。我们的研究结果表明，NAC、MYB、DOF、GATA 和 HD-ZIP 等 TF 家族高度参与种子发育过程。特别是，它们的优先伙伴选择以及基因表达谱的变化似乎与两个品种之间油含量的差异密切相关。这些发现对于增强我们对两个品种的遗传程序的理解以及为进一步的实验研究提出新的假设至关重要。
