Parsa I
State University of New York, Health Science Center at Brooklyn 11203.
Cancer Res. 1988 Apr 15;48(8):2265-72.
Toward the identification of steps in the multiphasic process of human pancreas carcinogenesis we have developed a panel of monoclonal antibodies to normal and carcinogen-treated human pancreas cells. One of these, an IgG3 with strong affinity for a membrane-associated Mr 78,000 protein in fetal and adult parenchymal cells, was purified by high performance liquid chromatography, and used for the detection and characterization of tumorigenic stage in human pancreas carcinogenesis. This protein was present on the cell surface of human pancreas explants exposed to methylnitrosourea for up to 4 months and in nontumorigenic cell lines derived from these explants. It was absent in a morphologically transformed subpopulation of cells in explants treated with methylnitrosourea for longer than 4 months, in tumorigenic cell lines derived from these explants, and in primary carcinomas of human pancreas. The presence of this marker in normal pancreas adjacent to tumors, in hyperplastic cells induced by methylnitrosurea and in nontumorigenic cell lines suggests a correlation between the loss of this membrane-associated marker and cell tumorigenicity.
为了确定人类胰腺癌多阶段发生过程中的各个步骤，我们制备了一组针对正常和经致癌物处理的人类胰腺细胞的单克隆抗体。其中一种是IgG3，对胎儿和成人实质细胞中一种与膜相关的78,000分子量蛋白具有强亲和力，通过高效液相色谱法进行纯化，并用于检测和表征人类胰腺癌发生过程中的致瘤阶段。该蛋白存在于暴露于甲基亚硝基脲长达4个月的人类胰腺外植体的细胞表面以及源自这些外植体的非致瘤细胞系中。在用甲基亚硝基脲处理超过4个月的外植体的形态转化细胞亚群、源自这些外植体的致瘤细胞系以及人类胰腺原发性癌中则不存在。该标志物在肿瘤旁正常胰腺、甲基亚硝基脲诱导的增生细胞以及非致瘤细胞系中的存在表明，这种与膜相关的标志物的丧失与细胞致瘤性之间存在关联。
