Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China.
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China.
Animal. 2021 Mar;15(3):100167. doi: 10.1016/j.animal.2020.100167. Epub 2021 Jan 22.
Taurine has been considered as an essential nutrient for many aquaculture species. While dietary taurine supplementation is highly recommended, novelty studies on taurine metabolism in fish are needed. The present study aimed to provide insight into the molecular mechanisms involved in multiple metabolome changes in Nile tilapia (Oreochromis niloticus) by studying plasma metabolic profile changes in response to graded levels of dietary taurine supplementation. The analysis used proton nuclear magnetic resonance-based metabolomics. Four groups of tilapias were fed with four diets supplemented with 0.0, 0.4, 0.8 and 1.2% taurine for 84 days. Fish plasma was sampled at multiple time points to provide an accurate snapshot of specific metabolic profiles during growth. Under the effect of taurine supplementation, 21 and 12 metabolites in tilapia plasma shown significant changes in terms of time-dependence and diet-dependence, respectively. These metabolic changes in tilapia plasma were mainly associated with energy and amino acid metabolism, lipids, nucleotides and protein metabolism. The results indicate that 0.8% taurine supplementation could significantly improve the carbohydrate synthesis, protein digestion and absorption, and fat deposition of tilapia and thereby promoted growth and development of tilapia.
牛磺酸一直被认为是许多水产养殖物种的必需营养素。尽管强烈推荐在饮食中补充牛磺酸，但仍需要对鱼类牛磺酸代谢进行创新性研究。本研究旨在通过研究尼罗罗非鱼（Oreochromis niloticus）血浆代谢谱对不同水平的饮食牛磺酸补充的反应，来深入了解多种代谢组变化所涉及的分子机制。该分析采用基于质子核磁共振的代谢组学方法。四组罗非鱼分别用四种饲料喂养，其中四种饲料分别补充 0.0、0.4、0.8 和 1.2%的牛磺酸，持续 84 天。在生长过程中，多次采集鱼血浆样本，以提供特定代谢谱的准确快照。在牛磺酸补充的影响下，21 种和 12 种代谢物在罗非鱼血浆中的时间依赖性和饮食依赖性方面均发生显著变化。罗非鱼血浆中的这些代谢变化主要与能量和氨基酸代谢、脂质、核苷酸和蛋白质代谢有关。结果表明，0.8%的牛磺酸补充可显著提高罗非鱼的碳水化合物合成、蛋白质消化吸收和脂肪沉积能力，从而促进罗非鱼的生长发育。
