Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.
Antimicrob Agents Chemother. 2021 Mar 18;65(4). doi: 10.1128/AAC.02431-20.
A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation, whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. c-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We previously presented evidence that the ectopic expression of the phosphodiesterase YhjH in results in biofilm dispersal. More recently, however, evidence has been presented that the induction of native c-di-GMP phosphodiesterases does not lead to a dispersal of biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for the development of antibiofilm drugs. However, here, we demonstrate that the induction of the c-di-GMP phosphodiesterases PA2133 and BifA indeed results in the dispersal of biofilms in both a microtiter tray biofilm assay and a flow cell biofilm system.
十年来的研究表明，二鸟苷酸环化酶(c-di-GMP)分子在许多细菌中充当中央第二信使。高水平的 c-di-GMP 与生物膜形成有关，而低水平的 c-di-GMP 与浮游单细胞细菌的生活方式有关。c-di-GMP 由双鸟苷酸环化酶形成，并被特定的磷酸二酯酶降解。我们之前的研究证据表明，在 中异位表达磷酸二酯酶 YhjH 会导致生物膜分散。然而，最近有证据表明，诱导天然 c-di-GMP 磷酸二酯酶不会导致 生物膜的分散。后者的结果可能会阻碍将 c-di-GMP 信号作为开发抗生物膜药物的目标的尝试。然而，在这里，我们证明诱导 c-di-GMP 磷酸二酯酶 PA2133 和 BifA 确实会导致微滴定板生物膜测定和流动细胞生物膜系统中 生物膜的分散。
