Wachowicz B, Krajewski T, Przybylska M
Department of Biochemistry, University of Lódź, Poland.
Cell Biochem Funct. 1988 Jan;6(1):1-6. doi: 10.1002/cbf.290060102.
A high molecular weight glycoprotein (450,000) was obtained from thrombin-treated duck thrombocytes by barium citrate adsorption technique followed by heparin-agarose affinity chromatography. Amino acid composition (high number of acidic amino acids and cystine) as well as carbohydrate contents (1.3 per cent hexosamine, 0.9 per cent sialic acid and 1.5 per cent hexose) showed similarity to mammalian platelet thrombospondin.
通过柠檬酸钡吸附技术,随后进行肝素-琼脂糖亲和层析,从经凝血酶处理的鸭血小板中获得了一种高分子量糖蛋白(450,000)。氨基酸组成(酸性氨基酸和胱氨酸数量较多)以及碳水化合物含量(1.3%的己糖胺、0.9%的唾液酸和1.5%的己糖)显示与哺乳动物血小板凝血酶敏感蛋白相似。