State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, China.
Department of Hepatic-Biliary-Pancreatic Medicine, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Mikrochim Acta. 2021 Jan 26;188(2):50. doi: 10.1007/s00604-021-04712-5.
A controlled method to prepare glutathione-protected bimetallic gold-platinum nanoclusters (Au-PtNCs) has been established. The Au-PtNCs show either strong red (625 nm) or near-infrared (NIR, 805 nm) emission. Further characterizations indicated that the average particle size grows from 1.42 to 1.78 nm, the larger particles being responsible for the redshift of emission. The NIR emitted Au-PtNCs are applied as a novel ratiometric probe of Ag(I), which induces a new emission peak at ~635 nm and quenches the initial emission gradually. The determination shows very high selectivity toward Ag(I) among other metal ions. A limit of determination (10 nM) and the linear range (0.10 to 15 μM) are achieved, which is much lower than the EPA mandate of 0.46 μM for Ag(I) in drinking water. The response mechanism is attributed to the fact that the added Ag(I) has been reduced by the core of Au-PtNCs and deposited on the surface, which induces new fluorescence emission around 635 nm. In addition, the ratiometric method is feasible for Ag(I) determination in serum serum with good recovery (between 98.3% and 102.0%, n = 3), showing very high application potential. The present study provides a controlled method to prepare Au-PtNCs with strong red and NIR emission and supplies a novel NIR ratiometric probe of Ag(I). Schematic presentation of the controlled preparation of glutathione-protected bimetallic gold-platinum nanoclusters (Au-PtNCs) with either red or near-infrared (NIR) emission, and application in ratiometric detection of Ag(I) with high selectivity and sensitivity.
一种控制方法制备了谷胱甘肽保护的双金属金-铂纳米团簇（Au-PtNCs）。Au-PtNCs 表现出强红色（625nm）或近红外（NIR，805nm）发射。进一步的表征表明，平均粒径从 1.42nm 增长到 1.78nm，较大的颗粒导致发射红移。发射近红外的 Au-PtNCs 被用作 Ag（I）的新型比率探针，它在约 635nm 处诱导新的发射峰，并逐渐猝灭初始发射。测定显示，在其他金属离子中对 Ag（I）具有非常高的选择性。实现了非常低的检测限（10nM）和线性范围（0.10 至 15μM），远低于 EPA 规定的饮用水中 Ag（I）的限值 0.46μM。响应机制归因于添加的 Ag（I）被 Au-PtNCs 的核心还原并沉积在表面上，这在 635nm 左右诱导新的荧光发射。此外，比率法可用于血清中 Ag（I）的测定，回收率良好（98.3%至 102.0%，n=3），显示出非常高的应用潜力。本研究提供了一种控制方法来制备具有强红色和近红外发射的谷胱甘肽保护的双金属金-铂纳米团簇，并提供了一种新型的 Ag（I）比率探针，具有高选择性和灵敏度。
