State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, People's Republic of China.
Institute of Theoretical Chemistry, College of Chemistry, Jilin University, No. 2 Liutiao Road, Changchun, 130023, People's Republic of China.
Mikrochim Acta. 2023 Aug 31;190(9):374. doi: 10.1007/s00604-023-05955-0.
When thiolactic acid-capped gold nanoclusters (AuNCs@TLA) with strong near-infrared (NIR, 800 nm) emission were applied to detect metal ions, only Ag induced the generation of two new emission peaks at 610 and 670 nm in sequence and quenching the original NIR emission. The new peak at 670 nm generated after the 800-nm emission disappeared utterly. The ratiometric and turn-on responses showed different linear concentration ranges (0.10-4.0 μmol·L and 10-50 μmol·L) toward Ag, and the limit of detection (LOD) was 40 nmol·L. Especially, the probe exhibited extremely high selectivity and strong anti-interference from other metal ions. Mechanism studies showed that the novel responses were attributed to the anti-galvanic reaction of AuNCs to Ag and formation of bimetallic nanoclusters. The two new emission peaks were due to the composition change and size growth of the metal core. Besides, bovine serum albumin (BSA) has been employed as a signal amplifier based on the assembly-induced emission enhancement properties of AuNCs, which improved the LOD to 10 nmol·L. Moreover, the ratiometric method is feasible for Ag detection in diluted serum with high recovery rates, showing large application potential in the biological system. The present study supplies a novel ratiometric probe for Ag with a two-stage response and provides a novel signal amplifier of BSA, which will facilitate and promote the application of NIR-emitted metal nanoclusters in biological system.
当巯基乳酸修饰的金纳米簇（AuNCs@TLA）具有强近红外（NIR，800nm）发射时，用于检测金属离子，只有 Ag 依次诱导产生两个新的发射峰，分别在 610 和 670nm 处，并猝灭原始 NIR 发射。800nm 发射消失后，在 670nm 处生成的新峰完全消失。比率和开启响应显示出不同的线性浓度范围（0.10-4.0μmol·L 和 10-50μmol·L）对 Ag，检测限（LOD）为 40nmol·L。特别是，该探针表现出极高的选择性和对其他金属离子的强抗干扰性。机制研究表明，这种新的响应归因于 AuNCs 对 Ag 的反电偶反应和形成双金属纳米簇。两个新的发射峰是由于金属核的组成变化和尺寸生长。此外，牛血清白蛋白（BSA）已被用作信号放大器，基于 AuNCs 的组装诱导发射增强特性，将 LOD 提高到 10nmol·L。此外，比率法可用于高回收率的稀释血清中的 Ag 检测，在生物体系中具有很大的应用潜力。本研究为 Ag 提供了一种具有两阶段响应的新型比率探针，并提供了 BSA 的新型信号放大器，这将促进和推动近红外发射金属纳米簇在生物体系中的应用。
