Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada; Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada; Department of Laboratory Medicine, St. Joseph's Healthcare Hamilton, Hamilton, ON, Canada; M.G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, DeGroote School of Medicine, McMaster University, Hamilton, ON, Canada.
Diagn Microbiol Infect Dis. 2021 May;100(1):115295. doi: 10.1016/j.diagmicrobio.2020.115295. Epub 2020 Dec 24.
Epstein-Barr Virus (EBV) exposure and illness is common in undergraduate university students and may affect academic achievement, social life, and quality of life. We designed a study to measure EBV exposure (EBV-IgG, either Epstein-Barr nuclear antigen 1 (EBNA-1)-IgG or viral capsid antigen (VCA)-IgG) and current viral shedding (EBV-DNA) using self-collected oral swabs among university undergraduate students. Of 184 students enrolled, 129 (70.1%) tested positive for EBV-IgG. Salivopositivity was associated with being in a current relationship, but not with enrollment year. Forty (21.7%) of the participants tested positive for EBV-DNA, which was associated with all symptom scores, including history of sore throat, fever, swollen glands, muscle weakness, and fatigue in the previous 6 months. Our findings suggest that noninvasive, self-collected oral flocked swabs are feasible and potentially valuable for measuring EBV IgG antibodies and DNA.
爱泼斯坦-巴尔病毒（EBV）在大学生中很常见，可能会影响学业成绩、社交生活和生活质量。我们设计了一项研究，使用自我采集的口腔拭子来测量大学生的 EBV 暴露情况（EBV-IgG，包括 EBV 核抗原 1（EBNA-1）-IgG 或病毒衣壳抗原（VCA）-IgG）和当前的病毒脱落（EBV-DNA）。在招募的 184 名学生中，有 129 名（70.1%）检测出 EBV-IgG 阳性。唾液阳性与当前的恋爱关系有关，但与入学年份无关。40 名（21.7%）参与者 EBV-DNA 检测呈阳性，这与所有症状评分有关，包括过去 6 个月的喉咙痛、发热、腺体肿胀、肌肉无力和疲劳病史。我们的研究结果表明，非侵入性、自我采集的口腔植绒拭子是可行的，并且可能具有测量 EBV IgG 抗体和 DNA 的价值。
