Shulman Iliya, Ogurcov Sergei, Kostennikov Alexander, Rogozin Alexander, Garanina Ekaterina, Masgutova Galina, Sergeev Mikhail, Rizvanov Albert, Mukhamedshina Yana
Clinical Research Center for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Republic Clinical Hospital, 420138 Kazan, Russia.
Biology (Basel). 2021 Jan 24;10(2):87. doi: 10.3390/biology10020087.
Peripheral blood presents an available source of cells for both fundamental research and clinical use. In our study, we have evaluated the therapeutic potential of peripheral blood mononuclear cells (PBMCs) excluding the preliminary sorting or mobilization of peripheral blood stem cells. We have evaluated the regenerative potential of PBMCs embedded into a fibrin matrix (FM) in a model of pig spinal cord injury. The distribution of transplanted PBMCs in the injured spinal cord was evaluated; PBMCs were shown to penetrate into the deep layers of the spinal cord and concentrate mainly in the grey matter. The results of the current study revealed an increase in the tissue integrity in the area adjacent to the epicenter of injury and the partially restored conduction along posterior columns of the spinal cord in animals after FM+PBMC application. The multiplex analysis of blood serum and cerebrospinal fluid showed the cytokine imbalance to occur without significantly shifting toward pro-inflammatory or anti-inflammatory cytokine cascades.
外周血是基础研究和临床应用中可用的细胞来源。在我们的研究中，我们评估了外周血单个核细胞（PBMC）的治疗潜力，排除了外周血干细胞的初步分选或动员。我们在猪脊髓损伤模型中评估了嵌入纤维蛋白基质（FM）的PBMC的再生潜力。评估了移植的PBMC在损伤脊髓中的分布；结果显示PBMC可穿透至脊髓深层并主要集中在灰质中。当前研究结果表明，应用FM + PBMC后，动物损伤震中附近区域的组织完整性增加，脊髓后柱的传导部分恢复。血清和脑脊液的多重分析显示细胞因子失衡，但未明显倾向于促炎或抗炎细胞因子级联反应。
