Jirků Milan, Lhotská Zuzana, Frgelecová Lucia, Kadlecová Oldřiška, Petrželková Klára Judita, Morien Evan, Jirků-Pomajbíková Kateřina
Biology Centre, Czech Academy of Sciences, Institute of Parasitology, Branišovská 31, 370 05 České Budějovice, Czech Republic.
Department of Medical Biology, Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic.
Microorganisms. 2021 Jan 22;9(2):226. doi: 10.3390/microorganisms9020226.
Colonization by the benign tapeworm, , has been associated with a reduction in intestinal inflammation and changes in bacterial microbiota. However, the role of microbiota in the tapeworm anti-inflammatory effect is not yet clear, and the aim of this study was to determine whether disruption of the microflora during worm colonization can affect the course of intestinal inflammation. We added a phase for disrupting the intestinal microbiota using antibiotics to the experimental design for which we previously demonstrated the protective effect of . We monitored the immunological markers, clinical parameters, bacterial microbiota, and histological changes in the colon of rats. After a combination of colonization, antibiotics, and colitis induction, we had four differently affected experimental groups. We observed a different course of the immune response in each group, but no protective effect was found. Rats treated with colonization and antibiotics showed a strong induction of the Th2 response as well as a significant change in microbial diversity. The microbial results also revealed differences in the richness and abundance of some bacterial taxa, influenced by various factors. Our data suggest that interactions between the tapeworm and bacteria may have a major impact on its protective effect.
良性绦虫的定殖与肠道炎症减轻及细菌微生物群变化有关。然而，微生物群在绦虫抗炎作用中的作用尚不清楚，本研究的目的是确定在绦虫定殖过程中破坏微生物群是否会影响肠道炎症的进程。我们在先前证明其具有保护作用的实验设计中增加了一个使用抗生素破坏肠道微生物群的阶段。我们监测了大鼠结肠中的免疫标志物、临床参数、细菌微生物群和组织学变化。在定殖、抗生素处理和诱导结肠炎相结合后，我们得到了四个受不同影响的实验组。我们观察到每组的免疫反应过程不同，但未发现保护作用。接受定殖和抗生素处理的大鼠表现出Th2反应的强烈诱导以及微生物多样性的显著变化。微生物结果还揭示了受各种因素影响的一些细菌类群在丰富度和丰度上的差异。我们的数据表明，绦虫与细菌之间的相互作用可能对其保护作用产生重大影响。
