Dublin Jerome High School, Dublin, OH 43016, USA.
Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Mil Med. 2021 Jan 25;186(Suppl 1):486-490. doi: 10.1093/milmed/usaa357.
The current study was designed to test the potential role of recombinant human MG53 (rhMG53) protein on protecting against alkaline-induced corneal injury in mice.
A round filter paper with 2-mm diameter was soaked in 1 mol/L of NaOH solution. The mouse alkaline injury was generated by placing the filter paper directly on the cornea for 30 seconds and washed with 30-mL saline; 10 µL of rhMG53 solution (20 µg/mL) or saline control was topically administrated on the mouse corneas (twice per day for 10 days). Re-epithelialization was measured by fluorescein staining and imaged by a slit lamp equipped with a digital camera. Clinical neovascularization and opacity scores were measured every day after injury. Ten days after injury, mice were sacrificed and corneas were dissected out for flat mount staining of CD31 for neovascularization.
MG53 was present in both dog aqueous humor and human tears. mg53-/- corneas were more susceptible to alkaline-induced corneal injury. Topical treatment of rhMG53 improved re-epithelialization, suppressed neovascularization, and fibrosis induced by alkaline injury.
rhMG53 may be an effective means to treat corneal wounding.
本研究旨在测试重组人 MG53（rhMG53）蛋白在保护小鼠碱性诱导性角膜损伤中的潜在作用。
用 2 毫米直径的圆形滤纸浸泡在 1 摩尔/升的 NaOH 溶液中。通过将滤纸直接放在角膜上 30 秒并用 30 毫升生理盐水冲洗来产生小鼠碱性损伤；将 rhMG53 溶液（20 µg/mL）或生理盐水对照物 10 µL 局部施用于小鼠角膜（每天两次，共 10 天）。通过荧光素染色测量再上皮化，并通过配备数码相机的裂隙灯成像。损伤后每天测量临床新生血管形成和混浊评分。损伤后 10 天，处死小鼠并取出角膜进行 CD31 平置染色以检测新生血管形成。
MG53 存在于犬房水中和人泪液中。mg53-/-角膜对碱性诱导的角膜损伤更敏感。rhMG53 的局部治疗可改善碱性损伤引起的再上皮化、抑制新生血管形成和纤维化。
rhMG53 可能是治疗角膜创伤的有效手段。
