Scarlett S M, Sagström S, Sagulin G B, Roomans G M
Department of Ultrastructure Research, University of Stockholm, Sweden.
Exp Mol Pathol. 1988 Apr;48(2):206-15. doi: 10.1016/0014-4800(88)90057-3.
It has been suggested that a defective chloride transport is the primary cellular basis for the disease cystic fibrosis (CF). Therefore, the effects of chronic furosemide treatment on the structure and function of rat exocrine glands were investigated. X-ray microanalysis of the submandibular gland showed an increase in the cellular Ca and Mg concentrations, and a decrease in the cellular Cl concentration. Transmission electron microscopy showed intracellular accumulation of mucus and the presence of mucus in acinar and ductal lumina. The volume of saliva secreted by the submandibular gland after pilocarpine stimulation was markedly reduced in furosemide-treated animals; the salivary concentrations of Na and Ca were higher, and that of K was lower, than in control animals. The protein concentration in submandibular saliva was not significantly affected. The response of the submandibular gland to isoproterenol stimulation was reduced in furosemide-treated animals. In the parotid gland, chronic furosemide treatment caused an accumulation of immature zymogen granules in the acinar cells and a decrease in the cellular Cl concentration. In the pancreas, the acinar lumen was dilated and completely filled with secretory material, and the acinar cells contained less Na and somewhat less Cl than in control animals. The chronically furosemide-treated rat shows a number of parallels with other animal models for CF, in particular the chronically reserpinized rat. There is also agreement with the human disease itself.
有人提出，氯化物转运缺陷是囊性纤维化（CF）疾病的主要细胞基础。因此，研究了慢性速尿治疗对大鼠外分泌腺结构和功能的影响。对下颌下腺的X射线微分析显示细胞内钙和镁浓度增加，细胞内氯浓度降低。透射电子显微镜显示细胞内黏液积聚以及腺泡和导管腔内存在黏液。在速尿治疗的动物中，毛果芸香碱刺激后下颌下腺分泌的唾液量明显减少；与对照动物相比，唾液中钠和钙的浓度较高，钾的浓度较低。下颌下唾液中的蛋白质浓度没有受到显著影响。在速尿治疗的动物中，下颌下腺对异丙肾上腺素刺激的反应降低。在腮腺中，慢性速尿治疗导致腺泡细胞中未成熟的酶原颗粒积聚，细胞内氯浓度降低。在胰腺中，腺泡腔扩张并完全充满分泌物质，与对照动物相比，腺泡细胞中钠和氯的含量较少。慢性速尿治疗的大鼠与其他CF动物模型有许多相似之处，特别是慢性利血平化的大鼠。这也与人类疾病本身相符。
