Müller R M, Roomans G M
Scan Electron Microsc. 1985(Pt 4):1583-601.
Elemental distribution and ultrastructure of the submandibular gland, the parotid gland and the pancreas were investigated in three suggested animal models of the disease cystic fibrosis: the chronically reserpinized rat, the chronically isoproterenol-treated rat, and the chronically pilocarpine-treated rat. To elucidate the cellular mechanism underlying the effects of these treatments, chronic effects of specific alpha - and beta -adrenergic agonists, as well as acute effects of reserpine and various agonists were also investigated. Reserpine, isoproterenol, and pilocarpine cause an increase in the calcium concentration in submandibular gland acinar cells, due to an increased calcium content of the intracellular mucus. In the parotid gland, reserpine and isoproterenol cause a decrease of the calcium concentration in acinar cells, due to a lower calcium content of the zymogen granules. In the submandibular gland, a decreased cellular Na concentration was noted after chronic treatment with isoproterenol or pilocarpine, and after a single dose of reserpine or isoproterenol. Ultrastructural changes in the exocrine glands investigated included excessive accumulation of intracellular secretory material and formation of abnormal uncondensed secretion granules. A common pattern in the 'animal models' appears to be (1) inhibition of secretion resulting in intracellular accumulation of secretory material, (2) synthesis of secretory macromolecules with altered cation-binding properties.
在三种囊性纤维化疾病的动物模型中，即长期使用利血平的大鼠、长期使用异丙肾上腺素的大鼠和长期使用毛果芸香碱的大鼠，研究了下颌下腺、腮腺和胰腺的元素分布及超微结构。为阐明这些处理效果背后的细胞机制，还研究了特定α和β肾上腺素能激动剂的慢性作用以及利血平和各种激动剂的急性作用。利血平、异丙肾上腺素和毛果芸香碱会导致下颌下腺腺泡细胞内钙浓度升高，这是由于细胞内黏液中钙含量增加所致。在腮腺中，利血平和异丙肾上腺素会导致腺泡细胞内钙浓度降低，这是由于酶原颗粒中钙含量较低所致。在下颌下腺中，长期使用异丙肾上腺素或毛果芸香碱以及单次使用利血平或异丙肾上腺素后，细胞内钠浓度会降低。所研究的外分泌腺的超微结构变化包括细胞内分泌物质的过度积累以及异常未浓缩分泌颗粒的形成。“动物模型”中的一个常见模式似乎是：（1）分泌受到抑制，导致分泌物质在细胞内积累；（2）合成具有改变的阳离子结合特性的分泌大分子。
