Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining, China.
Phytochem Anal. 2021 Sep;32(5):794-803. doi: 10.1002/pca.3025. Epub 2021 Jan 26.
Arenaria kansuensis Maxim. (AKM) is one of the most valued medicinal and edible herbs widely used in Qinghai-Tibet Plateau and there is also a large number of AKM bioactive constituents for health benefits of human beings. However, few works have referred to phytochemical content, fingerprint analysis and quality control of AKM. Therefore, the establishment of validated analytical methods is urgently needed for fingerprint comparison and quantitative analysis of AKM multicomponent.
To determine quantitatively and compare the phytochemical constituents of AKM located at different areas.
The chemical constituents in AKM samples were separated, identified, and quantified by high-performance liquid chromatography (HPLC) with a diode array detector. The discrimination and separation models for the chemical constituents were developed by chemometric analysis.
The flavones and β-carboline alkaloids were rich in AKM herbs, and the overall pattern of phytochemical profiles was the same, while the significant differences were detected in the total flavonoids, total β-carboline alkaloids and individual contents, especially the predominant compounds such as tricin and arenarine B. This demonstrated that ecogeographical origin gave an important impact on phytochemical compositions which could be considered as reliable parameters for classifying the AKM resources. Moreover, the contents of AKM constituents were higher in July and/or August than other months of the year, and there were no significant differences in the main phytochemical contents between cultivated and wild AKM herbs.
This study could provide credible data and method for geographical origin trace, comprehensive evaluation and further utilization of AKM resources.
多裂委陵菜（AKM）是青藏高原地区最有价值的药用和食用草本植物之一，其含有大量生物活性成分，对人类健康有益。然而，关于 AKM 的植物化学物质含量、指纹图谱分析和质量控制的研究很少。因此，迫切需要建立经过验证的分析方法，以进行 AKM 多组分的指纹图谱比较和定量分析。
定量测定和比较不同产地 AKM 的植物化学物质组成。
采用高效液相色谱法（HPLC）结合二极管阵列检测器对 AKM 样品中的化学成分进行分离、鉴定和定量。采用化学计量学分析建立化学成分的判别和分离模型。
AKM 草本植物富含黄酮类和β-咔啉生物碱，其植物化学特征图谱整体相似，但总黄酮、总β-咔啉生物碱和个别成分的含量存在显著差异，特别是主要化合物如tricinand arenarine B。这表明生态地理起源对植物化学成分有重要影响，可作为 AKM 资源分类的可靠参数。此外，AKM 成分的含量在 7 月和/或 8 月较高，而栽培和野生 AKM 草本植物的主要植物化学物质含量无显著差异。
本研究可为 AKM 资源的地理起源追溯、综合评价和进一步利用提供可靠的数据和方法。
