Li Jian, Kong Jing, Li Shenglong, Zhao Yu, Zhao Yakun, Xiao Dongguang, Yu Aiqun
Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):130-141. doi: 10.13345/j.cjb.200225.
In recent years, adaptive laboratory evolution (ALE) has emerged as a powerful tool for basic research in microbiology (e.g., molecular mechanisms of microbial evolution) and efforts on evolutionary engineering of microbial strains (e.g., accelerated evolution of industrial strains by bringing beneficial mutations). The ongoing rapid development of next-generation sequencing platforms has provided novel insights into growth kinetics and metabolism of microbes, and thus led to great advances of this technique. In this review, we summarize recent advances in the applications of long-term and short-term ALE techniques mainly for microbial strain engineering, and different modes of ALE are also introduced. Furthermore, we discuss the current limitations of ALE and potential solutions. We believe that the information reviewed here will make a significant contribution to further advancement of ALE.
近年来，适应性实验室进化（ALE）已成为微生物学基础研究（如微生物进化的分子机制）以及微生物菌株进化工程（如通过引入有益突变加速工业菌株进化）的有力工具。下一代测序平台的持续快速发展为微生物的生长动力学和代谢提供了新的见解，从而推动了该技术的巨大进步。在本综述中，我们总结了长期和短期ALE技术主要在微生物菌株工程应用方面的最新进展，并介绍了ALE的不同模式。此外，我们讨论了ALE目前的局限性和潜在解决方案。我们相信，这里综述的信息将为ALE的进一步发展做出重大贡献。
