Dong Jinyun, Fang Fang, Zhang Jialing, Xu Runze, Weng Jiayu, Cao Jiashun
Key Laboratory of Integrates Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, Jiangsu, China.
Guohe Environmental Research Institute (Nanjing) Co., Ltd, Nanjing 210048, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):149-162. doi: 10.13345/j.cjb.200203.
Polyhydroxyalkanoates (PHA) synthesis by activated sludge using volatile fatty acids (VFAs) in fermentation liquid of excess sludge as carbon source is a hotspot in the field of environmental biotechnology. However, there is no unified conclusion on the effects of non-VFAs, mainly dissolved organic matter (DOM), on PHA production. Thus, this critical review mainly introduces the main characteristics and common analysis methods of DOM in anaerobic fermentation liquid. The effects of DOM on PHA production are analyzed from the aspects of microbiology, metabolic regulation and sludge properties. The results of different studies showed that high concentration of DOM is bad for PHA production, but an appropriate amount of DOM is conducive to the stability of sludge properties, reducing the final PHA purification cost. Finally, suitable strategies were proposed to regulate the PHA synthesis by activated sludge with DOM for PHA production by anaerobic fermentation liquid.
利用剩余污泥发酵液中的挥发性脂肪酸（VFAs）作为碳源，通过活性污泥合成聚羟基脂肪酸酯（PHA）是环境生物技术领域的一个研究热点。然而，关于非VFAs（主要是溶解性有机物，DOM）对PHA产量的影响，目前尚无统一结论。因此，本综述主要介绍了厌氧发酵液中DOM的主要特性和常用分析方法。从微生物学、代谢调控和污泥性质等方面分析了DOM对PHA产量的影响。不同研究结果表明，高浓度的DOM不利于PHA的产生，但适量的DOM有利于污泥性质的稳定，降低最终PHA的纯化成本。最后，提出了合适的策略来调控活性污泥与DOM合成PHA，以实现厌氧发酵液生产PHA。
