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[镉污染地区番茄品种筛选及其抗氧化能力研究]

[Screening of tomato cultivars in cadmium-polluted areas and study on their antioxidant capacity].

作者信息

Yang Jiamin, Bao Hexigeduleng, Wan Jiayue, Ding Yanfei, Wang Feijuan, Zhu Cheng

机构信息

Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):242-252. doi: 10.13345/j.cjb.200242.

Abstract

To screen the available tomato pollution-safe cultivar varieties and reduce the potential food safety risks in Cd-polluted areas, the differences of Cd accumulation in different tomato (Solanum lycopersicum) varieties in southern China were studied by soil culture and hydroponic experiments. Firstly, the high and low accumulation varieties were selected from 25 tomato varieties under 2.94 mg/kg Cd stress by soil culture test, and then the responses of high and low accumulation tomato varieties to Cd stress were determined by hydroponic experiments. The results of soil culture test show that under 2.94 mg/kg Cd stress, there were significant differences in plant height, total biomass and yield among 25 tomato cultivars, and the Cd contents of fruits of all 25 tomato cultivars exceeded the highest limit value (0.05 mg/kg) of CAC (Codex alimentarius commission). Through cluster analysis, 7, 4 and 14 varieties accumulating relatively high, medium, and low concentrations of Cd in the fruits were screened, among which the highest, the lowest, and the average Cd contents in the fruits were 3.06 mg/kg DW, 1.47 mg/kg DW, and 2.21 mg/kg DW, respectively. The results of hydroponic experiment show that under the same concentration of Cd stress, Qiantangxuri F1, a high Cd accumulating variety, absorbed Cd faster, accumulated more Cd, used shorter oxidative stress response time and had stronger tolerance to Cd than Zhefen 3053, a low Cd accumulating variety. The typical high and low Cd accumulating varieties can provide a reference for agricultural production in heavy metal polluted areas and the development of molecular-assisted breeding methods of PSC. At present, cultivating low Cd accumulating PSC varieties and dynamic monitoring of Cd contents in tomato fruits are feasible methods in medium and light Cd-polluted areas.

摘要

为筛选出适宜的番茄安全品种,降低镉污染地区潜在的食品安全风险,通过土培和水培试验研究了中国南方不同番茄品种镉积累差异。首先,通过土培试验在2.94mg/kg镉胁迫下从25个番茄品种中筛选出高积累和低积累品种,然后通过水培试验测定高积累和低积累番茄品种对镉胁迫的响应。土培试验结果表明,在2.94mg/kg镉胁迫下,25个番茄品种的株高、总生物量和产量存在显著差异,且25个番茄品种果实中的镉含量均超过了食品法典委员会(Codex alimentarius commission)的最高限量值(0.05mg/kg)。通过聚类分析,筛选出果实中镉积累相对较高、中等和较低浓度的品种分别为7个、4个和14个,其中果实中镉含量最高、最低和平均含量分别为3.06mg/kg DW、1.47mg/kg DW和2.21mg/kg DW。水培试验结果表明,在相同浓度镉胁迫下,高镉积累品种钱江旭日F1吸收镉速度更快,积累镉更多,氧化应激响应时间更短,对镉的耐受性比低镉积累品种浙粉3053更强。典型的高镉积累和低镉积累品种可为重金属污染地区的农业生产和低镉积累品种分子辅助育种方法的开发提供参考。目前,培育低镉积累品种并动态监测番茄果实中的镉含量是中轻度镉污染地区可行的方法。

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