Dikkeboom R, Tijnagel J M, van der Knaap W P
Laboratory of Medical Parasitology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Dev Comp Immunol. 1988 Winter;12(1):17-32. doi: 10.1016/0145-305x(88)90021-3.
The mouse monoclonal antibody LS1 recognizes a membrane epitope present on circulating hemocytes of the gastropod mollusc Lymnaea stagnalis. In both juvenile and adult pond snails, LS1+ (LS1 positive) hemocytes have the morphology of immature cells. The percentage of LS1+ hemocytes is higher in juveniles (ca. 39%) than it is in adults (ca. 14%). Functional characteristics of LS1+ hemocytes and lectin binding to these cells were studied. In both age groups, the proliferative activity, as measured by the incorporation of deoxybromouridine, is much higher for LS1+ hemocytes than it is for LS1- (LS1 negative) cells. LS1+ hemocytes are phagocytically less active and have a lower lysosomal enzyme (peroxidase) content as compared to hemocytes that lack the epitope. Histochemical staining of the total population of circulating hemocytes shows that the lectins DBA, BS-l-A4 and BS-l-B4, PNA, SBA and ECA do not react with the hemocytes. LTA, APA, WGA, Con A and LCA bind to all hemocytes. RCA and STA recognize surface carbohydrate moieties present on subpopulations of hemocytes only. The LS1+ hemocyte population virtually lacks the carbohydrate residues recognized by STA, whereas the LS1- population never shows binding of RCA. Our results support the findings that the LS1 epitope is a membrane marker of less differentiated hemocytes in both juvenile and adult L. stagnalis. Furthermore, they suggest a correlation between the presence of the LS1 epitope and the absence of STA binding, whereas absence of the LS1 marker may correlate with the presence of a sugar recognized by RCA.
小鼠单克隆抗体LS1识别存在于腹足纲软体动物椎实螺循环血细胞上的一种膜表位。在幼年和成年池塘螺中，LS1 +（LS1阳性）血细胞具有未成熟细胞的形态。幼年螺中LS1 +血细胞的百分比（约39%）高于成年螺（约14%）。研究了LS1 +血细胞的功能特性以及凝集素与这些细胞的结合情况。在两个年龄组中，通过脱氧溴尿苷掺入法测定，LS1 +血细胞的增殖活性比LS1 -（LS1阴性）细胞高得多。与缺乏该表位的血细胞相比，LS1 +血细胞的吞噬活性较低，溶酶体酶（过氧化物酶）含量也较低。循环血细胞总数的组织化学染色显示，凝集素DBA、BS-l-A4和BS-l-B4、PNA、SBA和ECA不与血细胞发生反应。LTA、APA、WGA、Con A和LCA与所有血细胞结合。RCA和STA仅识别血细胞亚群上存在的表面碳水化合物部分。LS1 +血细胞群体几乎缺乏STA识别的碳水化合物残基，而LS1 -群体从未显示出RCA的结合。我们的结果支持以下发现：LS1表位是幼年和成年椎实螺中分化程度较低的血细胞的膜标记。此外，它们表明LS1表位的存在与STA结合的缺失之间存在相关性，而LS1标记的缺失可能与RCA识别的一种糖的存在相关。
