来自非洲大蜗牛血淋巴的一种半乳糖特异性凝集素：纯化与特性分析。

A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization.

作者信息

Mitra D, Sarkar M

机构信息

Indian Institute of Chemical Biology, Ja davpur, Calcutta.

出版信息

Dev Comp Immunol. 1988 Winter;12(1):33-42. doi: 10.1016/0145-305x(88)90022-5.

DOI:10.1016/0145-305x(88)90022-5

PMID:3350191

Abstract

A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.

摘要

在福寿螺（Achatina fulica fulica Bowdich）的血淋巴中检测到一种半乳糖特异性凝集素。该凝集素能凝集兔红细胞，已通过在交联瓜尔胶上进行亲和色谱，随后在Biogel P - 200柱上进行凝胶过滤进行了纯化。纯化后的凝集素天然分子量为210,000，由分子量为15,000的非共价连接的相同亚基组成。发现该凝集素的pI为8.1。凝集素的活性与二价阳离子无关。血凝抑制数据表明，2 - 脱氧 - D - 半乳糖是该凝集素最有效的抑制剂，其次是半乳糖和乳糖。