Sarkar M, Bachhawat B K, Mandal C
Arch Biochem Biophys. 1984 Aug 15;233(1):286-9. doi: 10.1016/0003-9861(84)90627-1.
An electrophoretically homogeneous agglutinin was purified from the albumin gland of Achatina fulica snails using asialofetunin-Sepharose 4B as an affinity column. The agglutinin was found to be temperature sensitive; it agglutinated rabbit and human umbilical cord erythrocytes only at low temperature. It was found to be specific for methyl-beta-D-galactoside, and the best inhibitor was N-acetyllactosamine.
使用去唾液酸胎球蛋白-琼脂糖4B作为亲和柱,从褐云玛瑙螺的白蛋白腺中纯化出一种电泳均一的凝集素。发现该凝集素对温度敏感;它仅在低温下凝集兔和人脐带红细胞。发现它对甲基-β-D-半乳糖苷具有特异性,最佳抑制剂是N-乙酰乳糖胺。