基于氨基 BODIPY 的蓝色荧光探针用于醛脱氢酶 1 表达细胞。
Amino BODIPY-Based Blue Fluorescent Probes for Aldehyde Dehydrogenase 1-Expressing Cells.
机构信息
Kavli Institute for the Physics and Mathematics of the Universe (Kavli IPMU, WPI), The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8583, Japan.
Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, 6-5-1 Kashiwanoha, Kashiwa, Chiba 277-8577, Japan.
出版信息
Bioconjug Chem. 2021 Feb 17;32(2):234-238. doi: 10.1021/acs.bioconjchem.0c00565. Epub 2021 Jan 27.
Aldehyde dehydrogenase 1 (ALDH1) plays an important role as a stem cell marker. In the field of stem cell biology, a green fluorescent ALDH1 probe has been principally used, but there is a need for more options in probe color. We designed and synthesized two blue fluorescent ALDH1 probes using 8-amino BODIPY and aminomethylbenzaldehyde. These probes can be simultaneously used with other color probes. Here, we demonstrate successful examples of the simultaneous use of these probes with green fluorescent protein.
醛脱氢酶 1（ALDH1）作为干细胞标志物发挥着重要作用。在干细胞生物学领域，主要使用绿色荧光 ALDH1 探针，但在探针颜色方面需要更多的选择。我们使用 8-氨基 BODIPY 和氨甲基苯甲醛设计并合成了两种蓝色荧光 ALDH1 探针。这些探针可以与其他颜色的探针同时使用。在这里，我们展示了这些探针与绿色荧光蛋白同时使用的成功示例。
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