de Castro Isabela Coelho, Pequito Danielle Cristina Tonello, Borghetti Gina, Yamaguchi Adriana Aya, de Brito Gleisson Alisson Pereira, Yamazaki Ricardo Key, Pôrto Laura Cristina Jardim, Coimbra Terezila Machado, Fernandes Luiz Claudio, Fernandez Ricardo
Department of Nutrition, Federal University of Lavras, Lavras, Brazil.
Federal University of Mato Grosso do Sul (UFMS), Três Lagoas, Brazil.
Arch Physiol Biochem. 2023 Jun;129(3):810-820. doi: 10.1080/13813455.2021.1874019. Epub 2021 Jan 27.
Present study investigated which diet, high-carbohydrate (HCD) or high-fat (HFD), most effectively induces classical characteristics of obesity in mice. Mice were fed commercial chow (control), an HCD, or an HFD for 12weeks. HFD and HCD increased body weight, fat mass, and glycaemia, whereas the HFD augmented insulinemia. In the kidney, the HFD caused albuminuria, and reductions in fractional Na excretion, Thromboxane B2 (TXB2) excretion, and urinary flow, whereas the HCD reduced glomerular filtration, plasma osmolality, and TXB2 and Prostaglandin E2 excretion. The consumption of HFD and HCD modified parameters that indicate histopathological changes, such as proliferation (proliferating-cell-nuclear antigen), inflammation (c-Jun N-terminal-protein), and epithelial-mesenchymal transition (vimentin, and desmin) in renal tissue, but the HCD group presents fewer signals of glomerular hypertrophy or tubule degeneration. In summary, the HCD generated the metabolic and renal changes required for an obesity model, but with a delay in the development of these modifications concerning the HFD.
