Chen Da, Song Ziyi, Lian Meiling, Yang Yi, Lin Shen, Xiao Lehui
School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China.
Nanoscale. 2021 Feb 7;13(5):2914-2922. doi: 10.1039/d0nr08492a. Epub 2021 Jan 27.
Fibrinogen participates in many physiological processes and is a biomarker for a variety of diseases. On this account, the development of a sensitive method for fibrinogen assay is particularly important. Herein, we demonstrate a new color-coded single-particle detection (SPD) method for fibrinogen detection by using platelet membrane-coated fluorescent polystyrene nanoparticles (PNPs) as the probes. Due to the specific interactions between fibrinogen and integrin receptors on platelet membranes, PNPs can form aggregated structures in the presence of fibrinogen. Therefore, colocalization events between green and red PNPs and the corresponding Pearson's correlation coefficient vary with the concentrations of fibrinogen. The sensing ability shows a linear range of 30-300 μg mL and a limit of detection (LOD) of 3.9 μg mL (11.3 nM) for fibrinogen detection. Moreover, it has been validated that the proposed biosensor can selectively detect fibrinogen and shows a good performance in real sample applications.
纤维蛋白原参与许多生理过程，是多种疾病的生物标志物。因此，开发一种灵敏的纤维蛋白原检测方法尤为重要。在此，我们展示了一种新的颜色编码单颗粒检测（SPD）方法，用于通过使用血小板膜包被的荧光聚苯乙烯纳米颗粒（PNP）作为探针来检测纤维蛋白原。由于纤维蛋白原与血小板膜上整联蛋白受体之间的特异性相互作用，PNP在纤维蛋白原存在下可形成聚集结构。因此，绿色和红色PNP之间的共定位事件以及相应的皮尔逊相关系数随纤维蛋白原浓度而变化。该传感能力在30 - 300μg/mL范围内呈线性，纤维蛋白原检测的检测限（LOD）为3.9μg/mL（11.3 nM）。此外，已证实所提出的生物传感器能够选择性地检测纤维蛋白原，并且在实际样品应用中表现良好。
