Wang Peng, Di Xiao, Li Fengshi, Rong Zhihua, Lian Wenzhuo, Li Zongshu, Chen Tianqi, Wang Wenjing, Zhong Qing, Sun Guoqiang, Ni Leng, Liu ChangWei
Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuaifuyuan Hutong, Dongcheng District, Beijing 100730, China.
Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
ACS Appl Bio Mater. 2025 Jan 20;8(1):399-409. doi: 10.1021/acsabm.4c01373. Epub 2024 Dec 26.
Therapeutic angiogenesis has garnered significant attention as a potential treatment strategy for lower limb ischemic diseases. Although hepatocyte growth factor (HGF) has been identified as a key promoter of therapeutic angiogenesis, its clinical application is limited due to its short half-life. In this study, we successfully developed and characterized platelet membrane-coated HGF-poly(lactic--glycolic acid) (PLGA) nanoparticles (NPs). These nanoparticles demonstrated enhanced capabilities to promote endothelial cell (EC) proliferation, migration, and tube formation in vitro. Additionally, their efficacy in improving tissue perfusion and promoting angiogenesis was confirmed in a hindlimb ischemia rat model. Our findings suggest that platelet membrane-coated HGF-PLGA-NPs could serve as a promising therapeutic approach for enhancing angiogenesis and restoring tissue perfusion in ischemic conditions.
治疗性血管生成作为下肢缺血性疾病的一种潜在治疗策略已受到广泛关注。尽管肝细胞生长因子（HGF）已被确定为治疗性血管生成的关键促进因子，但其临床应用因其半衰期短而受到限制。在本研究中，我们成功开发并表征了血小板膜包被的HGF-聚乳酸-乙醇酸共聚物（PLGA）纳米颗粒（NPs）。这些纳米颗粒在体外表现出增强的促进内皮细胞（EC）增殖、迁移和血管生成的能力。此外，在大鼠后肢缺血模型中证实了它们在改善组织灌注和促进血管生成方面的功效。我们的研究结果表明，血小板膜包被的HGF-PLGA-NPs可能是一种有前景的治疗方法，可增强缺血条件下的血管生成并恢复组织灌注。
