Sakamoto Yuta, Niwa Masatoshi, Muramatsu Ken, Shimo Satoshi
Department of Physical Therapy, Faculty of Health Sciences, Health Science University, 7187 Kodachi, Fujikawaguchiko-machi, Minamitsuru-gun, Yamanashi 401-0380, Japan.
Graduate School of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo 181-8612, Japan.
Int J Mol Sci. 2021 Jan 25;22(3):1165. doi: 10.3390/ijms22031165.
Several studies highlighted that obesity and diabetes reduce immune function. However, changes in the distribution of immunoglobins (Igs), including immunoglobulin-A (IgA), that have an important function in mucosal immunity in the intestinal tract, are unclear. This study aimed to investigate the impaired immune functions in the context of a diet-induced obese murine model via the assessment of the Igs in the intestinal villi. We used mice fed a high-fat diet (HFD) from four to 12 or 20 weeks of age. The distributions of IgA, IgM, and IgG1 were observed by immunohistochemistry. Interestingly, we observed that IgA was immunolocalized in many cells of the lamina propria and that immunopositive cells increased in mice aged 12 to 20 weeks. Notably, mice fed HFD showed a reduced number of IgA-immunopositive cells in the intestinal villi compared to those fed standard chow. Of note, the levels of IgM and IgG1 were also reduced in HFD fed mice. These results provide insights into the impaired mucosal immune function arising from diet-induced obesity and type 2 diabetes.
多项研究强调肥胖和糖尿病会降低免疫功能。然而，包括免疫球蛋白A（IgA）在内的免疫球蛋白（Igs）分布的变化尚不清楚，而IgA在肠道黏膜免疫中具有重要功能。本研究旨在通过评估肠绒毛中的免疫球蛋白，在饮食诱导的肥胖小鼠模型中研究受损的免疫功能。我们使用从4周龄到12周龄或20周龄喂食高脂饮食（HFD）的小鼠。通过免疫组织化学观察IgA、IgM和IgG1的分布。有趣的是，我们观察到IgA在固有层的许多细胞中免疫定位，并且在12至20周龄的小鼠中免疫阳性细胞增加。值得注意的是，与喂食标准饲料的小鼠相比，喂食HFD的小鼠肠绒毛中IgA免疫阳性细胞数量减少。值得注意的是，喂食HFD的小鼠中IgM和IgG1水平也降低。这些结果为饮食诱导的肥胖和2型糖尿病引起的黏膜免疫功能受损提供了见解。
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