Suzuki Akihiko, Suzuki Miwa
Laboratory of Aquatic Animal Physiology, Department of Marine Science and Resources, Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-0880, Japan.
Microorganisms. 2021 Jan 25;9(2):243. doi: 10.3390/microorganisms9020243.
In the present study, we isolated and characterized (. ) subsp. from a female Cuvier's beaked whale () stranded in Shizuoka, Japan. Only five isolates (CBW1-5), grown on Lactobacilli de Man Rogosa Sharpe (MRS) agar plates prepared using 50% artificial seawater, were positive in . species-specific primer PCR. Their 16S rRNA sequences were highly similar to those of . subsp. JCM 5805. The Gram reaction, motility, gas production from glucose, catalase production, and growth conditions were consistent with those of the type strain. Additionally, carbohydrate utilization of the strains was consistent with previously reported marine organism-derived strains. The pH-neutralized cell-free culture supernatant of strain CBW2 inhibited the growth of subsp. ATCC 6051 and ATCC 17749, whereas protease treatment eliminated or diminished its inhibitory activity. The strain possesses a precursor of the nisin structural gene (), which showed 100% homology with nisin Z, and nisin biosynthesis-related genes (, , , , , , and ), suggesting that the strain produces a nisin-like substance. This study provides fundamental information on whale-derived . subsp. which may be useful for reducing the carriage of . subsp. and . .
在本研究中，我们从一头搁浅于日本静冈的雌性柯氏喙鲸（Ziphius cavirostris）中分离并鉴定了嗜盐放线菌（Actinopolyspora halophila）亚种。仅5株菌株（CBW1 - 5）在使用50%人工海水制备的德氏乳杆菌-罗格斯-夏普（MRS）琼脂平板上生长，并在嗜盐放线菌物种特异性引物PCR中呈阳性。它们的16S rRNA序列与嗜盐放线菌亚种嗜盐放线菌JCM 5805的序列高度相似。革兰氏反应、运动性、葡萄糖产气、过氧化氢酶产生以及生长条件均与模式菌株一致。此外，这些菌株的碳水化合物利用情况与先前报道的海洋生物来源菌株一致。菌株CBW2的pH中和无细胞培养上清液抑制了嗜盐放线菌亚种嗜盐放线菌ATCC 6051和嗜盐放线菌ATCC 17749的生长，而蛋白酶处理消除或降低了其抑制活性。该菌株拥有乳链菌肽结构基因（nisA）的前体，其与乳链菌肽Z显示出100%的同源性，以及乳链菌肽生物合成相关基因（nisB、nisC、nisI、nisP、nisR、nisT和nisFEG），表明该菌株产生一种类乳链菌肽物质。本研究提供了关于源自鲸鱼的嗜盐放线菌亚种的基础信息，这可能有助于减少嗜盐放线菌亚种和嗜盐放线菌的携带。
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